Results
author = "Wong, Chi-Huey"
The core trisaccharide of an N-linked glycoprotein intrinsically accelerates folding and enhances stability
Author(s) :
Hanson, Sarah R.
,
Culyba, Elizabeth K.
,
Hsu, Tsui-Ling
,
Wong, Chi-Huey
,
Kelly, Jeffery W.
,
Powers, Evan T.
Description :
The folding energetics of the mono-N-glycosylated adhesion domain of the human immune cell receptor cluster of differentiation 2 (hCD2ad) were studied systematically to understand the influence of the N-glycan on the folding energy landscape. Fully elaborated N-glycan structures accelerate folding b...
Repository :
Europe PubMed Central
Language(s) :
English
Extracellular sulfatases support cartilage homeostasis by regulating BMP and FGF signaling pathways
Author(s) :
Otsuki, Shuhei
,
Hanson, Sarah R.
,
Miyaki, Shigeru
,
Grogan, Shawn P.
,
Kinoshita, Mitsuo
,
Asahara, Hiroshi
,
Wong, Chi-Huey
Description :
The balance between anabolic and catabolic signaling pathways is critical in maintaining cartilage homeostasis and its disturbance contributes to joint diseases such as osteoarthritis (OA). A unique mechanism that modulates the activity of cell signaling pathways is controlled by extracellular hepar...
Repository :
Europe PubMed Central
Language(s) :
English
Altered Gag Polyprotein Cleavage Specificity of Feline Immunodeficiency Virus/Human Immunodeficiency Virus Mutant Proteases as Demonstrated in a Cell-Based Expression System
Author(s) :
Lin, Ying-Chuan
,
Brik, Ashraf
,
de Parseval, Aymeric
,
Tam, Karen
,
Torbett, Bruce E.
,
Wong, Chi-Huey
,
Elder, John H.
Description :
We have used feline immunodeficiency virus (FIV) protease (PR) as a mutational system to study the molecular basis of substrate-inhibitor specificity for lentivirus PRs, with a focus on human immunodeficiency virus type 1 (HIV-1) PR. Our previous mutagenesis studies demonstrated that discrete substi...
Repository :
Europe PubMed Central
Language(s) :
English
Targeting the carbohydrates on HIV-1: Interaction of oligomannose dendrons with human monoclonal antibody 2G12 and DC-SIGN
Author(s) :
Wang, Sheng-Kai
,
Liang, Pi-Hui
,
Astronomo, Rena D.
,
Hsu, Tsui-Ling
,
Hsieh, Shie-Liang
,
Burton, Dennis R.
,
Wong, Chi-Huey
Description :
It is widely accepted that the heavily glycosylated glycoprotein gp120 on the surface of HIV-1 shields peptide epitopes from recognition by the immune system and may promote infection in vivo by interaction with dendritic cells and transport to tissue rich in CD4+ T cells such as lymph nodes. A cons...
Repository :
Europe PubMed Central
Language(s) :
English
Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450BM3 demethylases
Author(s) :
Lewis, Jared C.
,
Bastian, Sabine
,
Bennett, Clay S.
,
Fu, Yu
,
Mitsuda, Yuuichi
,
Chen, Mike M.
,
Greenberg, William A.
Description :
Polysaccharides comprise an extremely important class of biopolymers that play critical roles in a wide range of biological processes, but the synthesis of these compounds is challenging because of their complex structures. We have developed a chemoenzymatic method for regioselective deprotection of...
Repository :
Europe PubMed Central
Language(s) :
English
Design of a potent CD1d-binding NKT cell ligand as a vaccine adjuvant
Author(s) :
Li, Xiangming
,
Fujio, Masakazu
,
Imamura, Masakazu
,
Wu, Douglass
,
Vasan, Sandhya
,
Wong, Chi-Huey
,
Ho, David D.
,
Tsuji, Moriya
Description :
The glycolipid α-galactosylceramide (α-GalCer) has been shown to bind CD1d molecules to activate invariant natural killer T (iNKT) cells, and subsequently induce activation of various immune-competent cells, including dendritic cells, thereby providing a significant adjuvant effect for various vacci...
Repository :
Europe PubMed Central
Language(s) :
English
Ganoderma lucidum Polysaccharides Induce Macrophage-Like Differentiation in Human Leukemia THP-1 Cells via Caspase and p53 Activation
Description :
Differentiation therapy by induction of tumor cells is an important method in the treatment of hematological cancers such as leukemia. Tumor cell differentiation ends cancer cells' immortality, thus stopping cell growth and proliferation. In our previous study, we found that fucose-containing polysa...
Repository :
Europe PubMed Central
Language(s) :
English
Survey of immune-related, mannose/fucose-binding C-type lectin receptors reveals widely divergent sugar-binding specificities
Author(s) :
Lee, Reiko T
,
Hsu, Tsui-Ling
,
Huang, Shau Ku
,
Hsieh, Shie-Liang
,
Wong, Chi-Huey
,
Lee, Yuan C
Description :
C-type lectins (CTLs) are proteins that contain one or more carbohydrate-recognition domains (CRDs) that require calcium for sugar binding and share high degree of sequence homology and tertiary structure. CTLs whose CRD contain EPN (Glu-Pro-Asn) tripeptide motifs have potential to bind mannose (Man...
Repository :
Europe PubMed Central
Language(s) :
English
The antioxidant protein alkylhydroperoxide reductase of Helicobacter pylori switches from a peroxide reductase to a molecular chaperone function
Author(s) :
Chuang, Ming-Hong
,
Wu, Ming-Shiang
,
Lo, Wan-Lin
,
Lin, Jaw-Town
,
Wong, Chi-Huey
,
Chiou, Shyh-Horng
Description :
Helicobacter pylori, an oxygen-sensitive microaerophilic bacterium, contains many antioxidant proteins, among which alkylhydroperoxide reductase (AhpC) is the most abundant. The function of AhpC is to protect H. pylori from a hyperoxidative environment by reduction of toxic organic hydroperoxides. W...
Repository :
Europe PubMed Central
Language(s) :
English
Evaluation of RNA-binding specificity of aminoglycosides with DNA microarrays
Author(s) :
Liang, Fu-Sen
,
Greenberg, William A.
,
Hammond, Jennifer A.
,
Hoffmann, Julia
,
Head, Steven R.
,
Wong, Chi-Huey
Description :
We have developed methods for using DNA array technology to probe the entire transcriptome to determine the RNA-binding specificity of ligands. Two methods were investigated. In the first method, the RNA-binding aminoglycoside antibiotic tobramycin was covalently linked to magnetic beads. The beads ...
Repository :
Europe PubMed Central
Language(s) :
English