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author = "Witholt, B"
<< < 1 2 3 4 > >>

Found 40 documents, displaying page 2 of 4

Fusion of small unilamellar vesicles with viable EDTA-treated Escherichia coli cells.

Author(s) : Marvin, H J , ter Beest, M B , Hoekstra, D , Witholt, B
Description : Fusion characteristics of EDTA-treated Escherichia coli cells with small unilamellar vesicles were investigated, using a membrane fusion assay based on resonance energy transfer. Ca2+-EDTA treatments of Escherichia coli O111:B4 (wild type), E. coli C600 (rough), and E. coli D21f2 (deep rough) which ...
Repository : Europe PubMed Central
Language(s) : English

Distribution of newly synthesized lipoprotein over the outer membrane and the peptidoglycan sacculus of an Escherichia coli lac-lpp strain.

Author(s) : Hiemstra, H , Nanninga, N , Woldringh, C L , Inouye, M , Witholt, B
Description : The insertion of newly synthesized lipoprotein molecules into the cell wall of Escherichia coli was studied topographically by immunoelectron microscopy. Lipoprotein was briefly induced with isopropyl-beta-D-thiogalactopyranoside in cells carrying lac-lpp on a low-copy-number plasmid in an E. coli l...
Repository : Europe PubMed Central
Language(s) : English

High-sensitivity detection of newly induced LamB protein on the Escherichia coli cell surface.

Author(s) : Vos-Scheperkeuter, G H , Hofnung, M , Witholt, B
Description : The kinetics of the appearance at the cell surface of the outer membrane LamB protein after induction were determined by using specific antibodies and radioiodinated protein A as a probe. This was done in two different induction systems. First, LamB protein was induced in a wild-type strain by the s...
Repository : Europe PubMed Central
Language(s) : English

Conversion of pBR322-based plasmids into broad-host-range vectors by using the Tn3 transposition mechanism.

Author(s) : Kok, M , Rekik, M , Witholt, B , Harayama, S
Description : We constructed a series of transposon vectors which allow efficient in vitro gene manipulation and subsequent introduction of cloned DNA into a variety of gram-negative bacteria. Transfer of the cloned fragment from these multicopy plasmids into self-transmissible broad-host-range vectors is achieve...
Repository : Europe PubMed Central
Language(s) : English

Release of outer membrane fragments from wild-type Escherichia coli and from several E. coli lipopolysaccharide mutants by EDTA and heat shock treatments.

Author(s) : Marvin, H J , ter Beest, M B , Witholt, B
Description : EDTA-induced outer membrane losses from whole cells of wild-type Escherichia coli (O111:B4) and several lipopolysaccharide (LPS) mutants derived from E. coli K-12 D21 were analyzed. EDTA treatment induced losses of LPS (up to 40%), outer membrane proteins OmpA, OmpF/C, and lipoprotein, periplasmic p...
Repository : Europe PubMed Central
Language(s) : English

Induction kinetics and cell surface distribution of Escherichia coli lipoprotein under lac promoter control.

Author(s) : Hiemstra, H , de Hoop, M J , Inouye, M , Witholt, B
Description : The induction kinetics and surface accessibility of the outer membrane lipoprotein were studied in an Escherichia coli strain with the lpp gene under control of the lac promoter. Free lipoprotein appeared rapidly after induction with isopropyl-beta-D-thiogalactopyranoside and reached a steady-state ...
Repository : Europe PubMed Central
Language(s) : English

Proceedings of International Symposium on Bacterial Polyhydroxyalkanoates

Author(s) : Eggink, G. , Steinbüchel, A. , Poirier, Y. , Witholt, B.
Description :
Repository : Wageningen University and Researchcenter Publications
Language(s) : Undetermined

Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers.

Author(s) : Huijberts, G N , Eggink, G , de Waard, P , Huisman, G W , Witholt, B
Description : The biosynthesis of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas putida KT2442 during growth on carbohydrates was studied. PHAs isolated from P. putida cultivated on glucose, fructose, and glycerol were found to have a very similar monomer composition. In addition to the major constituent 3-hydro...
Repository : Europe PubMed Central
Language(s) : English

Topography of the insertion of LamB protein into the outer membrane of Escherichia coli wild-type and lac-lamB cells.

Author(s) : Vos-Scheperkeuter, G H , Pas, E , Brakenhoff, G J , Nanninga, N , Witholt, B
Description : The appearance of newly induced LamB protein at the cell surface of Escherichia coli was followed topographically by immuno-electron microscopy. LamB protein was induced in E. coli wild-type or lac-lamB cells for a short period of time (4 to 6 min), such that the overall level of LamB protein in ind...
Repository : Europe PubMed Central
Language(s) : English

Production of Pseudomonas aeruginosa Rhamnolipid Biosurfactants in Heterologous Hosts

Author(s) : Ochsner, U. A. , Reiser, J. , Fiechter, A. , Witholt, B.
Description : The high-level production of rhamnolipid biosurfactants is a unique feature of Pseudomonas aeruginosa and is strictly regulated in response to environmental conditions. The final step in rhamnolipid biosynthesis is catalyzed by the rhlAB genes encoding a rhamnosyltransferase. The expression of the c...
Repository : Europe PubMed Central
Language(s) : English
<< < 1 2 3 4 > >>

Found 40 documents, displaying page 2 of 4