Results
author = "Vandekerckhove, J"
Proteomics of Arabidopsis Seed Germination : a Comparative Study of Wild-Type and Gibberellin-Deficient Seeds
Description :
We examined the role of gibberellins (GAs) in germination of Arabidopsis seeds by a proteomic approach. For that purpose, we used two systems. The first system consisted of seeds of the GA-deficient ga1 mutant, and the second corresponded to wild-type seeds incubated in paclobutrazol, a specific GA ...
Repository :
Wageningen University and Researchcenter Publications
Language(s) :
English
Comparative proteome analysis of Chlamydia trachomatis serovar A, D and L2
Author(s) :
Shaw, A.C.
,
Gevaert, K.
,
Demol, H.
,
Hoorelbeke, B.
,
Vandekerckhove, J.
,
Larsen, Martin Røssel
,
Roepstorff, P.
,
m.fl., no-firstname
Description :
Repository :
Research at ASB
Language(s) :
English
Characterization of a secreted Chlamydia protease
Author(s) :
Shaw, A.C.
,
Vandahl, B.B.
,
Larsen, M.R.
,
Roepstorff, P.
,
Gevaert, K.
,
Vandekerckhove, J.
,
m.fl., no-firstname
Description :
Repository :
Research at ASB
Language(s) :
English
Characterization of recombinant wild type and site-directed mutations of apolipoprotein C-III: Lipid binding, displacement of ApoE, and inhibition of lipoprotein lipase
Author(s) :
Liu, HQ
,
Talmud, PJ.
,
Lins, L
,
Brasseur, R.
,
Olivecrona, G
,
Peelman, F
,
Vandekerckhove, J.
,
Rosseneu, M
,
Labeur, C
Description :
The physicochemical properties of recombinant wild type and three site-directed mutants of apolipoprotein C-III (apoC-III), designed by molecular modeling to alter specific amino acid residues implicated in lipid binding (L9T/T20L, F64A/W65A) or LPL inhibition (K21A), were compared. Relative lipid b...
Repository :
DIAL - Dépôt Institutionnel de l'Académie Louvain
Language(s) :
English
A proposed architecture for lecithin cholesterol acyl transferase (LCAT): Identification of the catalytic triad and molecular modeling
Author(s) :
Peelman, F
,
Vinaimont, N
,
Verhee, A
,
Vanloo, B
,
Verschelde, JL
,
Labeur, C
,
Seguret-Mace, S
,
Duverger, N
,
Hutchinson, G
Description :
The enzyme cholesterol lecithin acyl transferase (LCAT) shares the Ser/Asp-Glu/His triad with lipases, esterases and proteases, but the low level of sequence homology between LCAT and these enzymes did not allow for the LCAT fold to be identified yet. We, therefore, relied upon structural homology c...
Repository :
DIAL - Dépôt Institutionnel de l'Académie Louvain
Language(s) :
English
Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715
Author(s) :
Höfte, H
,
de Greve, H
,
Seurinck, J
,
Jansens, S
,
Mahillon, Jacques
,
Ampe, C
,
Vandekerckhove, J
,
Vanderbruggen, H
,
Vanmontagu, M
Description :
A plasmid-encoded crystal protein gene (bt2) has been cloned from Bacillus thuringiensis berliner 1715. In Escherichia coli, it directs the synthesis of the 130-kDa protein (Bt2) which is toxic to larvae of Pieris brassicae and Manduca sexta. Comparison of the deduced amino acid sequence of this Bt2...
Repository :
DIAL - Dépôt Institutionnel de l'Académie Louvain
Language(s) :
English
A monoclonal anti-double-stranded DNA autoantibody binds to a 94-kDa cell-surface protein on various cell types via nucleosomes or a DNA-histone complex.
Author(s) :
Jacob, L
,
Viard, J P
,
Allenet, B
,
Anin, M F
,
Slama, F B
,
Vandekerckhove, J
,
Primo, J
,
Markovits, J
,
Jacob, F
,
Bach, J F
Description :
A crude supernatant of hybridoma secreting a monoclonal anti-double-stranded (ds)DNA antibody (PME77 mAb), used to stain fibroblasts (CVI cells) in immunofluorescence, gives a punctuated staining of variable intensity. We had suggested that anti-DNA antibodies bind to cell-surface protein(s) of seve...
Repository :
Europe PubMed Central
Language(s) :
English
Characterization of F107 fimbriae of Escherichia coli 107/86, which causes edema disease in pigs, and nucleotide sequence of the F107 major fimbrial subunit gene, fedA.
Author(s) :
Imberechts, H
,
De Greve, H
,
Schlicker, C
,
Bouchet, H
,
Pohl, P
,
Charlier, G
,
Bertschinger, H
,
Wild, P
,
Vandekerckhove, J
Description :
F107 fimbriae were isolated and purified from edema disease strain 107/86 of Escherichia coli. Plasmid pIH120 was constructed, which contains the gene cluster that codes for adhesive F107 fimbriae. The major fimbrial subunit gene, fedA, was sequenced. An open reading frame that codes for a protein w...
Repository :
Europe PubMed Central
Language(s) :
English
Isolation and nucleotide sequence of the F17-A gene encoding the structural protein of the F17 fimbriae in bovine enterotoxigenic Escherichia coli.
Author(s) :
Lintermans, P
,
Pohl, P
,
Deboeck, F
,
Bertels, A
,
Schlicker, C
,
Vandekerckhove, J
,
Van Damme, J
,
Van Montagu, M
,
De Greve, H
Description :
The genetic determinant for production of the fimbrial F17 adhesive antigen was isolated from a bovine enterotoxigenic Escherichia coli strain. The F17-A gene, coding for the structural component of the F17 fimbrial adhesin, was cloned and sequenced. An open reading frame of 540 base pairs encoding ...
Repository :
Europe PubMed Central
Language(s) :
English
Molecular cloning and structural analysis of a gene from Zea mays (L.) coding for a putative receptor for the plant hormone auxin.
Author(s) :
Hesse, T
,
Feldwisch, J
,
Balshüsemann, D
,
Bauw, G
,
Puype, M
,
Vandekerckhove, J
,
Löbler, M
,
Klämbt, D
,
Schell, J
,
Palme, K
Description :
The major auxin-binding protein from maize coleoptiles was purified to homogeneity. The protein has an apparent mol. wt of 22 kd and binds 1-naphthylacetic acid with a KD of 2.40 x 10(-7) M. Additional antigenically related proteins, present in very low amounts, could be demonstrated in maize coleop...
Repository :
Europe PubMed Central
Language(s) :
English