Results
author = "Singh, Prim B."
Epigenetic rejuvenation
Description :
Induced pluripotent stem (iPS) cells have provided a rational means of obtaining histo-compatible tissues for ‘patient-specific’ regenerative therapies (Hanna et al. 2010; Yamanaka & Blau 2010). Despite the obvious potential of iPS cell-based therapies, there are certain problems that must be overco...
Repository :
Europe PubMed Central
Language(s) :
English
Uncoupling global and fine-tuning replication timing determinants for mouse pericentric heterochromatin
Description :
Mouse chromocenters are clusters of late-replicating pericentric heterochromatin containing HP1 bound to trimethylated lysine 9 of histone H3 (Me3K9H3). Using a cell-free system to initiate replication within G1-phase nuclei, we demonstrate that chromocenters acquire the property of late replication...
Repository :
Europe PubMed Central
Language(s) :
English
Differential Subnuclear Localization and Replication Timing of Histone H3 Lysine 9 Methylation States
Description :
Mono-, di-, and trimethylation of specific histone residues adds an additional level of complexity to the range of histone modifications that may contribute to a histone code. However, it has not been clear whether different methylated states reside stably at different chromatin sites or whether the...
Repository :
Europe PubMed Central
Language(s) :
English
HP1 Recruits Activity-Dependent Neuroprotective Protein to H3K9me3 Marked Pericentromeric Heterochromatin for Silencing of Major Satellite Repeats
Author(s) :
Mosch, Kerstin
,
Franz, Henriette
,
Soeroes, Szabolcs
,
Singh, Prim B.
,
Fischle, Wolfgang
Description :
H3 lysine 9 trimethylation (H3K9me3) is a histone posttranslational modification (PTM) that has emerged as hallmark of pericentromeric heterochromatin. This constitutive chromatin domain is composed of repetitive DNA elements, whose transcription is differentially regulated. Mammalian cells contain ...
Repository :
Europe PubMed Central
Language(s) :
English
In Vivo Dynamics of Swi6 in Yeast: Evidence for a Stochastic Model of Heterochromatin†
Description :
The mechanism for transcriptional silencing of pericentric heterochromatin is conserved from fission yeast to mammals. Silenced genome regions are marked by epigenetic methylation of histone H3, which serves as a binding site for structural heterochromatin proteins. In the fission yeast Schizosaccha...
Repository :
Europe PubMed Central
Language(s) :
English
Transcriptional Regulation of Mouse Mast Cell Protease-2 by Interleukin-15*
Author(s) :
Mirghomizadeh, Farhad
,
Bullwinkel, Jörn
,
Orinska, Zane
,
Janssen, Ottmar
,
Petersen, Arnd
,
Singh, Prim B.
,
Bulfone-Paus, Silvia
Description :
Mast cells (MCs) play a critical role in innate and adaptive immunity through the release of cytokines, chemokines, lipid mediators, biogenic amines, and proteases. We recently showed that the activities of MC proteases are transcriptionally regulated by intracellularly retained interleukin-15 (IL-1...
Repository :
Europe PubMed Central
Language(s) :
English
Dynamic associations of heterochromatin protein 1 with the nuclear envelope
Author(s) :
Kourmouli, Niki
,
Theodoropoulos, Panayiotis A.
,
Dialynas, George
,
Bakou, Alexandra
,
Politou, Anastasia S.
,
Cowell, Ian G.
Description :
To study the dynamics of mammalian HP1 proteins we have microinjected recombinant forms of mHP1α, M31 and M32 into the cytoplasm of living cells. As could be expected from previous studies, the three fusion proteins were efficiently transported into the nucleus and targeted specific chromatin areas....
Repository :
Europe PubMed Central
Language(s) :
English
γH2AX Foci Form Preferentially in Euchromatin after Ionising-Radiation
Author(s) :
Cowell, Ian G.
,
Sunter, Nicola J.
,
Singh, Prim B.
,
Austin, Caroline A.
,
Durkacz, Barbara W.
,
Tilby, Michael J.
Description :
Repository :
Europe PubMed Central
Language(s) :
English
KAP-1 Corepressor Protein Interacts and Colocalizes with Heterochromatic and Euchromatic HP1 Proteins: a Potential Role for Krüppel-Associated Box–Zinc Finger Proteins in Heterochromatin-Mediated Gene Silencing
Author(s) :
Ryan, Robert F.
,
Schultz, David C.
,
Ayyanathan, Kasirajan
,
Singh, Prim B.
,
Friedman, Josh R.
,
Fredericks, William J.
Description :
Krüppel-associated box (KRAB) domains are present in approximately one-third of all human zinc finger proteins (ZFPs) and are potent transcriptional repression modules. We have previously cloned a corepressor for the KRAB domain, KAP-1, which is required for KRAB-mediated repression in vivo. To char...
Repository :
Europe PubMed Central
Language(s) :
English
Conservation of Heterochromatin Protein 1 Function
Author(s) :
Wang, Guozheng
,
Ma, Alicia
,
Chow, Cheok-man
,
Horsley, David
,
Brown, Nicholas R.
,
Cowell, Ian G.
,
Singh, Prim B.
Description :
Heterochromatin represents a cytologically visible state of heritable gene repression. In the yeast, Schizosaccharomyces pombe, the swi6 gene encodes a heterochromatin protein 1 (HP1)-like chromodomain protein that localizes to heterochromatin domains, including the centromeres, telomeres, and the d...
Repository :
Europe PubMed Central
Language(s) :
English