Results
author = "Pozzan, T"
Matrix alkalinization: a novel mitochondrial signal for sustained pancreatic β-cell activation
Author(s) :
Wiederkehr, Andreas
,
Park, Kyu-Sang
,
Dupont, Olivier
,
Demaurex, Nicolas
,
Pozzan, Tullio
,
Cline, Gary W
,
Wollheim, Claes B
Description :
Nutrient secretagogues activate mitochondria of the pancreatic β-cell through the provision of substrate, hyperpolarisation of the inner mitochondrial membrane and mitochondrial calcium rises. We report that mitochondrial matrix pH, a parameter not previously studied in the β-cell, also exerts an im...
Repository :
Europe PubMed Central
Language(s) :
English
Study of the intima-media thickening in carotid arteries of healthy elderly with high blood pressure and elderly with high blood pressure and dyslipidemia
Author(s) :
de Freitas, Elizabete Viana
,
Brandão, Andréa Araújo
,
Pozzan, Roberto
,
Magalhães, Maria Eliane
,
Castier, Márcia
Description :
Repository :
Europe PubMed Central
Language(s) :
English
Activators of protein kinase C depolarize insulin-secreting cells by closing K+ channels.
Description :
Carbohydrate stimuli of insulin secretion depolarize the pancreatic B cell and the B-cell line RINm5F by inhibiting ATP-sensitive K+ channels. We examined the possibility that this effect is mediated by activation of protein kinase C. In RINm5F cells, the triose D-glyceraldehyde evoked a rapid incre...
Repository :
Europe PubMed Central
Language(s) :
English
Internalization pathway of C3b receptors in human neutrophils and its transmodulation by chemoattractant receptors stimulation.
Author(s) :
Carpentier, J L
,
Lew, D P
,
Paccaud, J P
,
Gil, R
,
Iacopetta, B
,
Kazatchkine, M
,
Stendahl, O
,
Pozzan, T
Description :
On the surface of phagocytes, C3b receptors (CR1) bind C3b-coated particles and promote their ingestion after activation by appropriate stimuli such as lymphokines or the chemoattractant formyl methionyl leucyl phenylalanine (fMLP) and fibronectin. The aims of the present study were 1) to define at ...
Repository :
Europe PubMed Central
Language(s) :
English
"Calciosome," a cytoplasmic organelle: the inositol 1,4,5-trisphosphate-sensitive Ca2+ store of nonmuscle cells?
Author(s) :
Volpe, P
,
Krause, K H
,
Hashimoto, S
,
Zorzato, F
,
Pozzan, T
,
Meldolesi, J
,
Lew, D P
Description :
Calsequestrin (CS) is the protein responsible for the high-capacity, moderate affinity binding of Ca2+ within the terminal cisternae of the sarcoplasmic reticulum, believed up to now to be specific for striated muscle. The cells of two nonmuscle lines (HL-60 and PC12) and of two rat tissues (liver a...
Repository :
Europe PubMed Central
Language(s) :
English
H+/site, charge/site, and ATP/site ratios in mitochondrial electron transport
Description :
H+/site, charge/site, and ATP/site ratios have been determined at coupling sites I, II, and III. Three e- donors have been used for coupling site III: ferrocyanide, ascorbate + tetramethyl-p-phenylenediamine (TMPD), and succinate + TMPD. The H+/site ratios are 4.0 with ferrocyanide and 6.0 with succ...
Repository :
Europe PubMed Central
Language(s) :
English
Subcellular imaging of intramitochondrial Ca2+ with recombinant targeted aequorin: significance for the regulation of pyruvate dehydrogenase activity.
Author(s) :
Rutter, G A
,
Burnett, P
,
Rizzuto, R
,
Brini, M
,
Murgia, M
,
Pozzan, T
,
Tavaré, J M
,
Denton, R M
Description :
Specific targeting of the recombinant, Ca2+ -sensitive photoprotein, aequorin to intracellular organelles has provided new insights into the mechanisms of intracellular Ca2+ homeostasis. When applied to small mammalian cells, a major limitation of this technique has been the need to average the sign...
Repository :
Europe PubMed Central
Language(s) :
English
Reduced Loading of Intracellular Ca2+ Stores and Downregulation of Capacitative Ca2+Influx in Bcl-2–Overexpressing Cells
Author(s) :
Pinton, Paolo
,
Ferrari, Davide
,
Magalhães, Paulo
,
Schulze-Osthoff, Klaus
,
Di Virgilio, Francesco
,
Pozzan, Tullio
Description :
The mechanism of action of the oncogene bcl-2, a key regulator of the apoptotic process, is still debated. We have employed organelle-targeted chimeras of the Ca2+-sensitive photoprotein, aequorin, to investigate in detail the effect of Bcl-2 overexpression on intracellular Ca2+ homeostasis. In the ...
Repository :
Europe PubMed Central
Language(s) :
English
Glucose-stimulated insulin secretion correlates with changes in mitochondrial and cytosolic Ca2+ in aequorin-expressing INS-1 cells.
Author(s) :
Kennedy, E D
,
Rizzuto, R
,
Theler, J M
,
Pralong, W F
,
Bastianutto, C
,
Pozzan, T
,
Wollheim, C B
Description :
Nutrient-stimulated insulin secretion is dependent upon the generation of metabolic coupling factors in the mitochondria of the pancreatic B cell. To investigate the role of Ca2+ in mitochondrial function, insulin secretion from INS-1 cells stably expressing the Ca2+-sensitive photoprotein aequorin ...
Repository :
Europe PubMed Central
Language(s) :
English
Beat-to-beat oscillations of mitochondrial [Ca2+] in cardiac cells
Author(s) :
Robert, Valérie
,
Gurlini, Pamela
,
Tosello, Valeria
,
Nagai, Takeharu
,
Miyawaki, Atsushi
,
Di Lisa, Fabio
,
Pozzan, Tullio
Description :
The Ca2+-sensitive photoprotein aequorin and the new green fluorescent protein-based fluorescent Ca2+ indicators ‘ratiometric-pericam’ were selectively expressed in the mitochondria, cytosol and/or nucleus of spontaneously beating ventricular myocytes from neonatal rats. This combined strategy revea...
Repository :
Europe PubMed Central
Language(s) :
English