Results
author = "Mullenders, Leon H. F."
Clusters of transcription-coupled repair in the human genome
Author(s) :
Surrallés, Jordi
,
Ramírez, María J.
,
Marcos, Ricard
,
Natarajan, Adayapalam T.
,
Mullenders, Leon H. F.
Description :
A specialized nucleotide excision repair pathway known as transcription-coupled repair (TCR) counteracts the toxic effects of DNA damage in transcriptionally active genes. The clustering of active genes into gene-rich chromosomal domains predicts that the sites of TCR are unevenly distributed throug...
Repository :
Europe PubMed Central
Language(s) :
English
Association between transcriptional activity, local chromatin structure, and the efficiencies of both subpathways of nucleotide excision repair of melphalan adducts.
Author(s) :
Episkopou, Hara
,
Kyrtopoulos, Soterios A.
,
Sfikakis, Petros P.
,
Fousteri, Maria
,
Dimopoulos, Meletios A.
,
Mullenders, Leon H. F.
Description :
The repair of melphalan-induced N-alkylpurine monoadducts and interstrand cross-links was examined in different repair backgrounds, focusing on four genes (beta-actin, p53, N-ras, and delta-globin) with dissimilar transcription activities. Adducts were found to be substrates for both global genome r...
Repository :
ECNIS Repository (Environmental Cancer Risk, Nutrition and Individual Susceptibility Repository)
Language(s) :
English
Epidermal transit of replication-arrested, undifferentiated keratinocytes in UV-exposed XPC mice: An alternative to in situ apoptosis
Author(s) :
Stout, Gerdine J.
,
Westdijk, Daniel
,
Calkhoven, Dennis M.
,
Pijper, Olaf
,
Backendorf, Claude M. P.
,
Willemze, Rein
Description :
The interplay among nucleotide excision repair, cell-cycle regulation, and apoptosis in the UV-exposed epidermis is extremely important to avoid mutations and malignant transformation. In Xpc–/– mice deficient in global genome nucleotide excision repair (GGR), a cell-cycle arrest of epidermal cells ...
Repository :
Europe PubMed Central
Language(s) :
English
Domain structure, localization, and function of DNA polymerase η, defective in xeroderma pigmentosum variant cells
Author(s) :
Kannouche, Patricia
,
Broughton, Bernard C.
,
Volker, Marcel
,
Hanaoka, Fumio
,
Mullenders, Leon H.F.
,
Lehmann, Alan R.
Description :
DNA polymerase η carries out translesion synthesis past UV photoproducts and is deficient in xeroderma pigmentosum (XP) variants. We report that polη is mostly localized uniformly in the nucleus but is associated with replication foci during S phase. Following treatment of cells with UV irradiation ...
Repository :
Europe PubMed Central
Language(s) :
English
Differential role of transcription-coupled repair in UVB-induced G2 arrest and apoptosis in mouse epidermis
Author(s) :
van Oosten, Marijke
,
Rebel, Heggert
,
Friedberg, Errol C.
,
van Steeg, Harry
,
van der Horst, Gijsbertus T. J.
,
van Kranen, Henk J.
Description :
Nucleotide excision repair (NER), apoptosis, and cell-cycle
regulation are major defense mechanisms against the carcinogenic
effects of UVB light. NER eliminates UVB-induced DNA photolesions via
two subpathways: global genome repair (GGR) and transcription-coupled
repair (TCR). Defects in NER re...
Repository :
Europe PubMed Central
Language(s) :
English
Restoration of Nucleotide Excision Repair in a Helicase-Deficient XPD Mutant from Intragenic Suppression by a Trichothiodystrophy Mutation
Author(s) :
George, James W.
,
Salazar, Edmund P.
,
Vreeswijk, Maaike P. G.
,
Lamerdin, Jane E.
,
Reardon, Joyce T.
,
Zdzienicka, Malgorzata Z.
Description :
The UV-sensitive V-H1 cell line has a T46I substitution mutation in the Walker A box in both alleles of XPD and lacks DNA helicase activity. We characterized three partial revertants that curiously display intermediate UV cytotoxicity (2- to 2.5-fold) but normal levels of UV-induced hprt mutations. ...
Repository :
Europe PubMed Central
Language(s) :
English
Cellular Concentrations of DDB2 Regulate Dynamic Binding of DDB1 at UV-Induced DNA Damage▿
Author(s) :
Alekseev, Sergey
,
Luijsterburg, Martijn S.
,
Pines, Alex
,
Geverts, Bart
,
Mari, Pierre-Olivier
,
Giglia-Mari, Giuseppina
Description :
Nucleotide excision repair (NER) is the principal pathway for counteracting cytotoxic and mutagenic effects of UV irradiation. To provide insight into the in vivo regulation of the DNA damage recognition step of global genome NER (GG-NER), we constructed cell lines expressing fluorescently tagged da...
Repository :
Europe PubMed Central
Language(s) :
English
UV-induced inhibition of transcription involves repression of transcription initiation and phosphorylation of RNA polymerase II
Author(s) :
Rockx, Davy A. P.
,
Mason, Rebecca
,
van Hoffen, Anneke
,
Barton, Michelle Craig
,
Citterio, Elisabetta
,
Bregman, David B.
Description :
Cells from patients with Cockayne syndrome (CS) are hypersensitive to DNA-damaging agents and are unable to restore damage-inhibited RNA synthesis. On the basis of repair kinetics of different types of lesions in transcriptionally active genes, we hypothesized previously that impaired transcription ...
Repository :
Europe PubMed Central
Language(s) :
English
Separate Domains of Rev1 Mediate Two Modes of DNA Damage Bypass in Mammalian Cells▿ †
Author(s) :
Jansen, Jacob G.
,
Tsaalbi-Shtylik, Anastasia
,
Hendriks, Giel
,
Gali, Himabindu
,
Hendel, Ayal
,
Johansson, Fredrik
Description :
The Y family DNA polymerase Rev1 has been proposed to play a regulatory role in the replication of damaged templates. To elucidate the mechanism by which Rev1 promotes DNA damage bypass, we have analyzed the progression of replication on UV light-damaged DNA in mouse embryonic fibroblasts that conta...
Repository :
Europe PubMed Central
Language(s) :
English
Replication Factor C Recruits DNA Polymerase δ to Sites of Nucleotide Excision Repair but Is Not Required for PCNA Recruitment▿
Author(s) :
Overmeer, René M.
,
Gourdin, Audrey M.
,
Giglia-Mari, Ambra
,
Kool, Hanneke
,
Houtsmuller, Adriaan B.
,
Siegal, Gregg
Description :
Nucleotide excision repair (NER) operates through coordinated assembly of repair factors into pre- and postincision complexes. The postincision step of NER includes gap-filling DNA synthesis and ligation. However, the exact composition of this NER-associated DNA synthesis complex in vivo and the dyn...
Repository :
Europe PubMed Central
Language(s) :
English