Results
author = "Modesti, Mauro"
Plasma levels of matrix metalloproteinases 2 and 9 correlate with histological grade in breast cancer patients
Author(s) :
VASATURO, FORTUNATA
,
SOLAI, FABIANA
,
MALACRINO, CAROLINA
,
NARDO, TIZIANA
,
VINCENZI, BRUNO
,
MODESTI, MAURO
,
SCARPA, SUSANNA
Description :
Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in the process of tumor invasion and metastasis that are found throughout tissues and also in the plasma. The aim of this study was to investigate whether the evaluation of plasma concentrations of MMPs 2, 3 and 9 may have clinical si...
Repository :
Europe PubMed Central
Language(s) :
English
Revealing the competition between peeled ssDNA, melting bubbles, and S-DNA during DNA overstretching using fluorescence microscopy
Author(s) :
King, Graeme A.
,
Gross, Peter
,
Bockelmann, Ulrich
,
Modesti, Mauro
,
Wuite, Gijs J. L.
,
Peterman, Erwin J. G.
Description :
Mechanical stress plays a key role in many genomic processes, such as DNA replication and transcription. The ability to predict the response of double-stranded (ds) DNA to tension is a cornerstone of understanding DNA mechanics. It is widely appreciated that torsionally relaxed dsDNA exhibits a stru...
Repository :
Europe PubMed Central
Language(s) :
English
Dissecting Elastic Heterogeneity along DNA Molecules Coated Partly with Rad51 Using Concurrent Fluorescence Microscopy and Optical Tweezers
Author(s) :
Mameren, Joost van
,
Modesti, Mauro
,
Kanaar, Roland
,
Wyman, Claire
,
Wuite, Gijs J. L.
,
Peterman, Erwin J. G.
Description :
Nucleoprotein filament formation by recombinases is central to homologous recombination. To follow this process, we used fluorescent human Rad51 recombinase to visualize the interactions with double-stranded DNA (dsDNA). Fluorescence imaging revealed that Rad51 filament formation on dsDNA initiates ...
Repository :
Europe PubMed Central
Language(s) :
English
The structure-specific endonuclease Mus81–Eme1 promotes conversion of interstrand DNA crosslinks into double-strands breaks
Author(s) :
Hanada, Katsuhiro
,
Budzowska, Magda
,
Modesti, Mauro
,
Maas, Alex
,
Wyman, Claire
,
Essers, Jeroen
,
Kanaar, Roland
Description :
Repair of interstrand crosslinks (ICLs) requires multiple-strand incisions to separate the two covalently attached strands of DNA. It is unclear how these incisions are generated. DNA double-strand breaks (DSBs) have been identified as intermediates in ICL repair, but enzymes responsible for produci...
Repository :
Europe PubMed Central
Language(s) :
English
Domain swapping between FEN-1 and XPG defines regions in XPG that mediate nucleotide excision repair activity and substrate specificity
Author(s) :
Hohl, Marcel
,
Dunand-Sauthier, Isabelle
,
Staresincic, Lidija
,
Jaquier-Gubler, Pascale
,
Thorel, Fabrizio
,
Modesti, Mauro
Description :
FEN-1 and XPG are members of the FEN-1 family of structure-specific nucleases, which share a conserved active site. FEN-1 plays a central role in DNA replication, whereas XPG is involved in nucleotide excision repair (NER). Both FEN-1 and XPG are active on flap structures, but only XPG cleaves bubbl...
Repository :
Zurich Open Repository and Archive (ZORA)
Language(s) :
English
DNA-binding and strand-annealing activities of human Mre11: implications for its roles in DNA double-strand break repair pathways
Author(s) :
de Jager, Martijn
,
Dronkert, Mies L. G.
,
Modesti, Mauro
,
Beerens, Cecile E.
M. T.
,
Kanaar, Roland
,
van Gent, Dik
C.
Description :
DNA double-strand breaks (DSBs) in eukaryotic cells can be repaired
by non-homologous end-joining or homologous recombination. The complex
containing the Mre11, Rad50 and Nbs1 proteins has been implicated
in both DSB repair pathways, even though they are mechanistically
different. To get a bette...
Repository :
Europe PubMed Central
Language(s) :
English
Unraveling the structure of DNA during overstretching by using multicolor, single-molecule fluorescence imaging
Author(s) :
van Mameren, Joost
,
Gross, Peter
,
Farge, Geraldine
,
Hooijman, Pleuni
,
Modesti, Mauro
,
Falkenberg, Maria
,
Wuite, Gijs J. L.
Description :
Single-molecule manipulation studies have revealed that double-stranded DNA undergoes a structural transition when subjected to tension. At forces that depend on the attachment geometry of the DNA (65 pN or 110 pN), it elongates ≈1.7-fold and its elastic properties change dramatically. The nature of...
Repository :
Europe PubMed Central
Language(s) :
English
XRCC4's interaction with XLF is required for coding (but not signal) end joining
Author(s) :
Roy, Sunetra
,
Andres, Sara N.
,
Vergnes, Alexandra
,
Neal, Jessica A.
,
Xu, Yao
,
Yu, Yaping
,
Lees-Miller, Susan P.
,
Junop, Murray
Description :
XRCC4 and XLF are structurally related proteins important for DNA Ligase IV function. XRCC4 forms a tight complex with DNA Ligase IV while XLF interacts directly with XRCC4. Both XRCC4 and XLF form homodimers that can polymerize as heterotypic filaments independently of DNA Ligase IV. Emerging struc...
Repository :
Europe PubMed Central
Language(s) :
English
Domain swapping between FEN-1 and XPG defines regions in XPG that mediate nucleotide excision repair activity and substrate specificity
Author(s) :
Hohl, Marcel
,
Dunand-Sauthier, Isabelle
,
Staresincic, Lidija
,
Jaquier-Gubler, Pascale
,
Thorel, Fabrizio
,
Modesti, Mauro
Description :
FEN-1 and XPG are members of the FEN-1 family of structure-specific nucleases, which share a conserved active site. FEN-1 plays a central role in DNA replication, whereas XPG is involved in nucleotide excision repair (NER). Both FEN-1 and XPG are active on flap structures, but only XPG cleaves bubbl...
Repository :
Europe PubMed Central
Language(s) :
English
Conformational changes in CLIP-170 regulate its binding to microtubules and dynactin localization
Author(s) :
Lansbergen, Gideon
,
Komarova, Yulia
,
Modesti, Mauro
,
Wyman, Claire
,
Hoogenraad, Casper C.
,
Goodson, Holly V.
,
Lemaitre, Régis P.
Description :
Cytoplasmic linker protein (CLIP)-170, CLIP-115, and the dynactin subunit p150Glued are structurally related proteins, which associate specifically with the ends of growing microtubules (MTs). Here, we show that down-regulation of CLIP-170 by RNA interference results in a strongly reduced accumulati...
Repository :
Europe PubMed Central
Language(s) :
English