Results
author = "Martial, Joseph"
The Addition of Nine Residues at the C-Terminus of Human Prolactin Drastically Alters Its Biological Properties
Description :
We have added nine extra residues to the C-terminal of human prolactin and analysed the effect of this mutation on the ability of the hormone to bind to its lactogenic receptor and to induce Nb2 cell division. Both properties are markedly affected when compared to the natural 23-kDa human prolactin....
Repository :
ORBi (University of Liège)
Language(s) :
English
Evidence for a Second Receptor Binding Site on Human Prolactin
Description :
The existence of a second receptor binding site on human prolactin (hPRL) was investigated by site-directed mutagenesis. First, 12 residues of helices 1 and 3 were mutated to alanine. Since none of the resulting mutants exhibit reduced bioactivity in the Nb2 cell proliferation bioassay, the mutated ...
Repository :
ORBi (University of Liège)
Language(s) :
English
Expression of the zinc finger Egr1 gene during zebrafish embryonic development
Description :
Egr1 is a highly conserved zinc finger protein which plays important roles in many aspects of vertebrate development and in the adult. The cDNA coding for zebrafish Egr1 was obtained and its expression pattern was examined during zebrafish embryogenesis using whole-mount in situ hybridization. Egr1 ...
Repository :
ORBi (University of Liège)
Language(s) :
English
Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element
Description :
We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a f...
Repository :
ORBi (University of Liège)
Language(s) :
English
A transformed fish cell line expressing a green fluorescent protein-luciferase fusion gene responding to cellular stress
Description :
We obtained a stable transformed fish (EPC) cell line containing a reporter gene under the control of the tilapia HSP70 promoter. Expression of the reporter gene, coding for a green fluorescent protein (GFP)-luciferase fusion protein, was assessed by measuring the luciferase enzymatic activity by lu...
Repository :
ORBi (University of Liège)
Language(s) :
English
Cloning and expression of the TALE superclass homeobox Meis2 gene during zebrafish embryonic development
Author(s) :
Biemar, Frédéric
,
Devos, Nathalie
,
Martial, Joseph
,
Driever, Wolfgang
,
Peers, Bernard
Description :
Meis and Prep/Pknox (MEINOX family) proteins, together with Pbx (PBC family) proteins, belong to the TALE superfamily characterized by an atypical homeodomain containing three additional amino acids between helix 1 and helix 2. Members of the MEINOX and PBC families have been isolated in Caenorhabdi...
Repository :
ORBi (University of Liège)
Language(s) :
English
Sprouty1 , a new target of the angiostatic agent 16K prolactin, negatively regulates angiogenesis
Author(s) :
Sabatel Céline
,
Cornet Anne M
,
Tabruyn Sébastien P
,
Malvaux Ludovic
,
Castermans Karolien
,
Martial Joseph A
,
Struman Ingrid
Description :
Abstract
Background
Disorganized angiogenesis is associated with several pathologies, including cancer. The identification of new genes that control tumor neovascularization can provide novel insights for future anti-cancer therapies.
Repository :
DOAJ-Articles
Language(s) :
English
The antiangiogenic factor, 16-kDa human prolactin, induces endothelial cell cycle arrest by acting at both the G(0)-G(1) and the G(2)-M phases
Author(s) :
Tabruyn, Sébastien
,
Nguyen, Ngoc-Quynh-Nhu
,
Cornet, Anne
,
Martial, Joseph
,
Struman, Ingrid
Description :
The 16-kDa N-terminal fragment of human prolactin (16K hPRL) is a potent antiangiogenic factor that has been shown to prevent tumor growth in a xenograph mouse model. In this paper we first demonstrate that 16K hPRL inhibits serum-induced DNA synthesis in adult bovine aortic endothelial cells. This ...
Repository :
ORBi (University of Liège)
Language(s) :
English
Expression of the antiangiogenic factor 16K hPRL in human HCT116 colon cancer cells inhibits tumor growth in Rag1(-/-) mice
Description :
The M(r) 16,000 NH(2)-terminal fragment of human prolactin (16K hPRL) is a potent antiangiogenic factor inhibiting endothelial cell function in vitro and neovascularization in vivo. The present study was undertaken to test the ability of 16K hPRL to inhibit the growth of human HCT116 colon cancer ce...
Repository :
ORBi (University of Liège)
Language(s) :
English
Inhibition of urokinase activity by the antiangiogenic factor 16K prolactin: activation of plasminogen activator inhibitor 1 expression
Description :
The N-terminal fragment of PRL (16K PRL) is an antiangiogenic factor that, in vitro, inhibits several components of angiogenesis including basic fibroblast growth factor (bFGF)-induced cell division, migration, and organization of capillary endothelial cells. An essential step in the regulation of a...
Repository :
ORBi (University of Liège)
Language(s) :
English