Results
author = "Maréchal, V"
Mapping of the p53 and mdm-2 interaction domains.
Description :
The 90-kDa cellular protein encoded by the mouse mdm-2 oncogene binds to the p53 protein in vivo and inhibits its transactivation function (J. Momand, G. P. Zambetti, D. C. Olson, D. George, and A. J. Levine, Cell 69:1237-1245, 1992). cDNA clones encoding the human homolog of the mdm-2 protein (also...
Repository :
Europe PubMed Central
Language(s) :
English
Continuous systemic secretion of a lysosomal enzyme by genetically modified mouse skin fibroblasts.
Description :
Lysosomal enzymes secreted or externally supplied into the extracellular medium can be internalized by cells and targeted to lysosomes after binding to specific membrane receptors. This process allows for the replacement of the missing enzyme activity in deficient cells. Using a retroviral vector, w...
Repository :
UCL Eprints
Language(s) :
English
Disappearance of lysosomal storage in spleen and liver of mucopolysaccharidosis VII mice after transplantation of genetically modified bone marrow cells.
Description :
Mice homozygous for the gusmps allele lack beta-glucuronidase activity and provide a useful model for human Mucopolysaccharidosis type VII (MPS VII), also known as Sly syndrome. Bone marrow (BM) transplantation was shown to correct the metabolic defect and to increase the life span of diseased anima...
Repository :
UCL Eprints
Language(s) :
English
Human immunodeficiency virus type 1 Nef increases the efficiency of reverse transcription in the infected cell.
Description :
We have analyzed the replication of Nef+ and Nef- isogenic human immunodeficiency virus in CEM, HUT78, MT4 lymphoid, and U937 monocytic cell lines. At each passage of infected cells, we have assessed the relative infectivity of the virus particles released in culture media by measuring the number of...
Repository :
Europe PubMed Central
Language(s) :
English
The ribosomal L5 protein is associated with mdm-2 and mdm-2-p53 complexes.
Description :
Throughout the purification of the mdm-2 or mdm-2-p53 protein complexes, a protein with a molecular weight of 34,000 was observed to copurify with these proteins. Several monoclonal antibodies directed against distinct epitopes in the mdm-2 or p53 protein coimmunoprecipitated this 34,000-molecular-w...
Repository :
Europe PubMed Central
Language(s) :
English
Gene transfer to somatic tissues using retroviral vectors.
Description :
Retroviral vectors have successfully been used for stable gene transfer in a number of in vivo situation. Before this approach can reallistically be applied in the treatment of human genetic diseases, the correction of pathological symptoms have to be documented in an animal model. In this perspecti...
Repository :
UCL Eprints
Language(s) :
English
The DNA-binding domain of two bZIP transcription factors, the Epstein-Barr virus switch gene product EB1 and Jun, is a bipartite nuclear targeting sequence.
Description :
The Epstein-Barr virus BZLF1 gene product EB1 (also called ZEBRA and Zta), is a transcription factor belonging to the bZIP (basic domain leucine zipper) family of nuclear proteins. Translocation to the nucleus of EB1 (J. Becker, U. Leser, M. Marschall, A. Langford, W. Jilg, H. Gelderblom, P. Reichar...
Repository :
Europe PubMed Central
Language(s) :
English
Study of mother-to-child Epstein-Barr virus transmission by means of nested PCRs.
Description :
The aim of this study was to investigate the possibility of vertical Epstein-Barr virus (EBV) transmission. We developed two nested-PCR methods for amplifying distinct regions of EBV DNA (BNRF1 and BamHI W) in circulating lymphocytes. Nested PCR was applied to samples obtained from 67 mother-infant ...
Repository :
Europe PubMed Central
Language(s) :
English
Human immunodeficiency virus type 1 Nef induces accumulation of CD4 in early endosomes.
Author(s) :
Schwartz, O
,
Dautry-Varsat, A
,
Goud, B
,
Maréchal, V
,
Subtil, A
,
Heard, JM
,
Danos, O
Description :
We have studied the fate of CD4 in CEM T cells expressing a human immunodeficiency virus type 1 HIV-1 Nef protein. Nef triggered a rapid endocytosis and a degradation of CD4, while most of the p56lck was upheld at the cell membrane. In the presence of Nef, CD4 accumulated in acidic intracellular ves...
Repository :
UCL Eprints
Language(s) :
English
Validation of single real-time TaqMan PCR assay for the detection and quantitation of four major genotypes of hepatitis E virus in clinical specimens.
Description :
Since the characterization of the genome of the hepatitis E virus (HEV) in 1990, a large genetic diversity has been described. A single real-time reverse transcription (RT)-PCR assay with TaqMan technology has been validated which uses only one set of primers and probe within the ORF2 HEV region (nt...
Repository :
Oxford University Research Archive (ORA)
Language(s) :
English