Results
author = "Lin, Ying-Chuan"
Molecular Basis for the Relative Substrate Specificity of Human Immunodeficiency Virus Type 1 and Feline Immunodeficiency Virus Proteases
Description :
We have used a random hexamer phage library to delineate similarities and differences between the substrate specificities of human immunodeficiency virus type 1 (HIV-1) and feline immunodeficiency virus (FIV) proteases (PRs). Peptide sequences were identified that were specifically cleaved by each p...
Repository :
Europe PubMed Central
Language(s) :
English
Generation of Infectious Feline Immunodeficiency Virus (FIV) Encoding FIV/Human Immunodeficiency Virus Chimeric Protease▿
Description :
Feline immunodeficiency virus (FIV) and human immunodeficiency virus type 1 (HIV-1) proteases (PRs) share only 23% amino acid identity and exhibit distinct specificities yet have very similar 3-dimensional structures. Chimeric PRs in which HIV residues were substituted in structurally equivalent pos...
Repository :
Europe PubMed Central
Language(s) :
English
Crystal structure of an FIV/HIV chimeric protease complexed with the broad-based inhibitor, TL-3
Author(s) :
Heaslet Holly
,
Lin Ying-Chuan
,
Tam Karen
,
Torbett Bruce E
,
Elder John H
,
Stout C David
Description :
Abstract
We have obtained the 1.7 Å crystal structure of FIV protease (PR) in which 12 critical residues around the active site have been substituted with the structurally equivalent residues of HIV PR (12X FIV PR). The chimeric PR was crystallized in complex with the broad-based inhibitor...
Repository :
DOAJ-Articles
Language(s) :
English
Structural Basis for Distinctions between Substrate and Inhibitor Specificities for Feline Immunodeficiency Virus and Human Immunodeficiency Virus Proteases
Author(s) :
Lin, Ying-Chuan
,
Beck, Zachary
,
Morris, Garrett M.
,
Olson, Arthur J.
,
Elder, John H.
Description :
We used feline immunodeficiency virus (FIV) protease (PR) as a mutational framework to define determinants for the observed substrate and inhibitor specificity distinctions between FIV and human immunodeficiency virus (HIV) PRs. Multiple-substitution mutants were constructed by replacing the residue...
Repository :
Europe PubMed Central
Language(s) :
English
Crystal structure of an FIV/HIV chimeric protease complexed with the broad-based inhibitor, TL-3
Author(s) :
Heaslet, Holly
,
Lin, Ying-Chuan
,
Tam, Karen
,
Torbett, Bruce E
,
Elder, John H
,
Stout, C David
Description :
We have obtained the 1.7 Å crystal structure of FIV protease (PR) in which 12 critical residues around the active site have been substituted with the structurally equivalent residues of HIV PR (12X FIV PR). The chimeric PR was crystallized in complex with the broad-based inhibitor TL-3, which inhibi...
Repository :
Europe PubMed Central
Language(s) :
English
Viral Evolution in Response to the Broad-Based Retroviral Protease Inhibitor TL-3†
Author(s) :
Bühler, Bernd
,
Lin, Ying-Chuan
,
Morris, Garrett
,
Olson, Arthur J.
,
Wong, Chi-Huey
,
Richman, Douglas D.
,
Elder, John H.
Description :
TL-3 is a protease inhibitor developed using the feline immunodeficiency virus protease as a model. It has been shown to efficiently inhibit replication of human, simian, and feline immunodeficiency viruses and therefore has broad-based activity. We now demonstrate that TL-3 efficiently inhibits the...
Repository :
Europe PubMed Central
Language(s) :
English
Alteration of Substrate and Inhibitor Specificity of Feline Immunodeficiency Virus Protease
Author(s) :
Lin, Ying-Chuan
,
Beck, Zachary
,
Lee, Taekyu
,
Le, Van-Duc
,
Morris, Garrett M.
,
Olson, Arthur J.
,
Wong, Chi-Huey
,
Elder, John H.
Description :
Feline immunodeficiency virus (FIV) protease is structurally very similar to human immunodeficiency virus (HIV) protease but exhibits distinct substrate and inhibitor specificities. We performed mutagenesis of subsite residues of FIV protease in order to define interactions that dictate this specifi...
Repository :
Europe PubMed Central
Language(s) :
English
Altered Gag Polyprotein Cleavage Specificity of Feline Immunodeficiency Virus/Human Immunodeficiency Virus Mutant Proteases as Demonstrated in a Cell-Based Expression System
Author(s) :
Lin, Ying-Chuan
,
Brik, Ashraf
,
de Parseval, Aymeric
,
Tam, Karen
,
Torbett, Bruce E.
,
Wong, Chi-Huey
,
Elder, John H.
Description :
We have used feline immunodeficiency virus (FIV) protease (PR) as a mutational system to study the molecular basis of substrate-inhibitor specificity for lentivirus PRs, with a focus on human immunodeficiency virus type 1 (HIV-1) PR. Our previous mutagenesis studies demonstrated that discrete substi...
Repository :
Europe PubMed Central
Language(s) :
English
Assessment of FIV-C infection of cats as a function of treatment with the protease inhibitor, TL-3
Author(s) :
de Rozières, Sohela
,
Swan, Christina H
,
Sheeter, Dennis A
,
Clingerman, Karen J
,
Lin, Ying-Chuan
,
Huitron-Resendiz, Salvador
Description :
Repository :
Europe PubMed Central
Language(s) :
English
Structural basis for drug and substrate specificity exhibited by FIV encoding a chimeric FIV/HIV protease
Author(s) :
Lin, Ying-Chuan
,
Perryman, Alexander L.
,
Olson, Arthur J.
,
Torbett, Bruce E.
,
Elder, John H.
,
Stout, C. David
Description :
Crystal structures of the 6s-98S FIV protease chimera with darunavir and lopinavir bound have been determined at 1.7 and 1.8 Å resolution, respectively.
Repository :
Europe PubMed Central
Language(s) :
English