Results
author = "Kuhn, Peter"
Cyclic adenosine monophosphate differentiated β-endorphin neurons promote immune function and prevent prostate cancer growth
Author(s) :
Sarkar, Dipak K.
,
Boyadjieva, Nadka I.
,
Chen, Cui Ping
,
Ortigüela, María
,
Reuhl, Kenneth
,
Clement, E. Michael
,
Kuhn, Peter
Description :
Pituitary adenylate cyclase-activating peptide (PACAP), a cAMP-activating agent, is highly expressed in the hypothalamus during the period when many neuroendocrine cells become differentiated from the neural stem cells (NSCs). Activation of the cAMP system in rat hypothalamic NSCs differentiated the...
Repository :
Europe PubMed Central
Language(s) :
English
Supramolecular Architecture of Severe Acute Respiratory Syndrome Coronavirus Revealed by Electron Cryomicroscopy†
Author(s) :
Neuman, Benjamin W.
,
Adair, Brian D.
,
Yoshioka, Craig
,
Quispe, Joel D.
,
Orca, Gretchen
,
Kuhn, Peter
,
Milligan, Ronald A.
Description :
Coronavirus particles are enveloped and pleomorphic and are thus refractory to crystallization and symmetry-assisted reconstruction. A novel methodology of single-particle image analysis was applied to selected virus features to obtain a detailed model of the oligomeric state and spatial relationshi...
Repository :
Europe PubMed Central
Language(s) :
English
Nuclear Magnetic Resonance Structure of the Nucleic Acid-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus Nonstructural Protein 3▿
Author(s) :
Serrano, Pedro
,
Johnson, Margaret A.
,
Chatterjee, Amarnath
,
Neuman, Benjamin W.
,
Joseph, Jeremiah S.
,
Buchmeier, Michael J.
Description :
The nuclear magnetic resonance (NMR) structure of a globular domain of residues 1071 to 1178 within the previously annotated nucleic acid-binding region (NAB) of severe acute respiratory syndrome coronavirus nonstructural protein 3 (nsp3) has been determined, and N- and C-terminally adjoining polype...
Repository :
Europe PubMed Central
Language(s) :
English
Rastering strategy for screening and centring of microcrystal samples of human membrane proteins with a sub-10 µm size X-ray synchrotron beam
Author(s) :
Cherezov, Vadim
,
Hanson, Michael A.
,
Griffith, Mark T.
,
Hilgart, Mark C.
,
Sanishvili, Ruslan
,
Nagarajan, Venugopalan
Description :
Crystallization of human membrane proteins in lipidic cubic phase often results in very small but highly ordered crystals. Advent of the sub-10 µm minibeam at the APS GM/CA CAT has enabled the collection of high quality diffraction data from such microcrystals. Herein we describe the challenges and ...
Repository :
Europe PubMed Central
Language(s) :
English
Optical Quantification of Cellular Mass, Volume, and Density of Circulating Tumor Cells Identified in an Ovarian Cancer Patient
Author(s) :
Phillips, Kevin G.
,
Velasco, Carmen Ruiz
,
Li, Julia
,
Kolatkar, Anand
,
Luttgen, Madelyn
,
Bethel, Kelly
,
Duggan, Bridgette
Description :
Clinical studies have demonstrated that circulating tumor cells (CTCs) are present in the blood of cancer patients with known metastatic disease across the major types of epithelial malignancies. Recent studies have shown that the concentration of CTCs in the blood is prognostic of overall survival ...
Repository :
Europe PubMed Central
Language(s) :
English
Quantification of cellular volume and sub-cellular density fluctuations: comparison of normal peripheral blood cells and circulating tumor cells identified in a breast cancer patient
Author(s) :
Phillips, Kevin G.
,
Kolatkar, Anand
,
Rees, Kathleen J.
,
Rigg, Rachel
,
Marrinucci, Dena
,
Luttgen, Madelyn
,
Bethel, Kelly
Description :
Cancer metastasis, the leading cause of cancer-related deaths, is facilitated in part by the hematogenous transport of circulating tumor cells (CTCs) through the vasculature. Clinical studies have demonstrated that CTCs circulate in the blood of patients with metastatic disease across the major type...
Repository :
Europe PubMed Central
Language(s) :
English
Site-specific DNA-antibody conjugates for specific and sensitive immuno-PCR
Author(s) :
Kazane, Stephanie A.
,
Sok, Devin
,
Cho, Edward H.
,
Uson, Maria Loressa
,
Kuhn, Peter
,
Schultz, Peter G.
,
Smider, Vaughn V.
Description :
Antibody conjugates are widely used as diagnostics and imaging reagents. However, many such conjugates suffer losses in sensitivity and specificity due to nonspecific labeling techniques. We have developed methodology to site-specifically conjugate oligonucleotides to antibodies containing a genetic...
Repository :
Europe PubMed Central
Language(s) :
English
Nuclear Magnetic Resonance Structure of the N-Terminal Domain of Nonstructural Protein 3 from the Severe Acute Respiratory Syndrome Coronavirus▿
Author(s) :
Serrano, Pedro
,
Johnson, Margaret A.
,
Almeida, Marcius S.
,
Horst, Reto
,
Herrmann, Torsten
,
Joseph, Jeremiah S.
Description :
This paper describes the structure determination of nsp3a, the N-terminal domain of the severe acute respiratory syndrome coronavirus (SARS-CoV) nonstructural protein 3. nsp3a exhibits a ubiquitin-like globular fold of residues 1 to 112 and a flexibly extended glutamic acid-rich domain of residues 1...
Repository :
Europe PubMed Central
Language(s) :
English
Proteomics Analysis Unravels the Functional Repertoire of Coronavirus Nonstructural Protein 3▿ †
Author(s) :
Neuman, Benjamin W.
,
Joseph, Jeremiah S.
,
Saikatendu, Kumar S.
,
Serrano, Pedro
,
Chatterjee, Amarnath
,
Johnson, Margaret A.
Description :
Severe acute respiratory syndrome (SARS) coronavirus infection and growth are dependent on initiating signaling and enzyme actions upon viral entry into the host cell. Proteins packaged during virus assembly may subsequently form the first line of attack and host manipulation upon infection. A compl...
Repository :
Europe PubMed Central
Language(s) :
English
A mouse model for nonsyndromic deafness (DFNB12) links hearing loss to defects in tip links of mechanosensory hair cells
Author(s) :
Schwander, Martin
,
Xiong, Wei
,
Tokita, Joshua
,
Lelli, Andrea
,
Elledge, Heather M.
,
Kazmierczak, Piotr
,
Sczaniecka, Anna
Description :
Deafness is the most common form of sensory impairment in humans and is frequently caused by single gene mutations. Interestingly, different mutations in a gene can cause syndromic and nonsyndromic forms of deafness, as well as progressive and age-related hearing loss. We provide here an explanation...
Repository :
Europe PubMed Central
Language(s) :
English