Results
author = "Keith, W.N."
A gene expression signature classifying telomerase and ALT immortalization reveals an hTERT regulatory network and suggests a mesenchymal stem cell origin for ALT
Author(s) :
Lafferty-Whyte, K
,
Cairney, C J
,
Will, M B
,
Serakinci, N
,
Daidone, M-G
,
Zaffaroni, N
,
Bilsland, A
,
Keith, W N
Description :
Udgivelsesdato: 2009-Oct-29
,
Telomere length is maintained by two known mechanisms, the activation of telomerase or alternative lengthening of telomeres (ALT). The molecular mechanisms regulating the ALT phenotype are poorly understood and it is unknown how the decision of which pathway to activate is...
Repository :
Research at ASB
Language(s) :
English
Synthetic Anticancer Gene Medicine Exploits Intrinsic Antitumor Activity of Cationic Vector to Cure Established Tumors
Author(s) :
Dufes, C.
,
Keith, W.N.
,
Bilsland, A.
,
Proutski, I.
,
Uchegbu, I.F.
,
Schatzlein, A.G.
Description :
The systemic delivery of genetic therapies required for the treatment of inaccessible tumors and metastases remains a challenge despite the development of various viral and synthetic vector systems. Here we show that a synthetic vector system based on polypropylenimine dendrimers has the desired pro...
Repository :
Pharmacy Eprints
Language(s) :
Undetermined
Identification of genetic changes associated with drug resistance by reverse in situ hybridization.
Author(s) :
Hoare, S. F.
,
Freeman, C. A.
,
Coutts, J. C.
,
Varley, J. M.
,
James, L.
,
Keith, W. N.
Description :
The molecular cytogenetic techniques of comparative genomic hybridization (CGH) and reverse in situ hybridization (REVISH) allow the entire genomes of tumours to be screened for genetic changes without the requirement for specific probes or markers. In order to define the ability of REVISH to detect...
Repository :
Europe PubMed Central
Language(s) :
English
Aberrant expression of minichromosome maintenance proteins 2 and 5, and Ki-67 in dysplastic squamous oesophageal epithelium and Barrett's mucosa
Description :
Background: Minichromosome maintenance (Mcm) proteins are essential for eukaryotic DNA replication, and their expression implies potential for cell proliferation. Expression is dysregulated in dysplastic states but data for oesophageal squamous mucosa and Barrett's mucosa have not been published.
Repository :
Glasgow ePrints Service
Language(s) :
Undetermined
Selection of a subpopulation with fewer DNA topoisomerase II alpha gene copies in a doxorubicin-resistant cell line panel.
Author(s) :
Withoff, S.
,
Keith, W. N.
,
Knol, A. J.
,
Coutts, J. C.
,
Hoare, S. F.
,
Mulder, N. H.
,
de Vries, E. G.
Description :
A panel of doxorubicin-resistant sublines of the human small-cell lung carcinoma cell line GLC4 displays decreasing DNA topoisomerase II alpha (TopoII alpha) mRNA levels with increasing resistance. In the present study we describe how this decrease may be regulated. No significant differences in Top...
Repository :
Europe PubMed Central
Language(s) :
English
Differential expression of DNA topoisomerase II alpha and -beta in P-gp and MRP-negative VM26, mAMSA and mitoxantrone-resistant sublines of the human SCLC cell line GLC4.
Author(s) :
Withoff, S.
,
de Vries, E. G.
,
Keith, W. N.
,
Nienhuis, E. F.
,
van der Graaf, W. T.
,
Uges, D. R.
,
Mulder, N. H.
Description :
Sublines of the human small-cell lung carcinoma (SCLC) cell line GLC4 with acquired resistance to teniposide, amsacrine and mitoxantrone (GLC4/VM20x, GLC4/AM3x and GLC4/MIT60x, respectively) were derived to study the contribution of DNA topoisomerase II alpha and -beta (TopoII alpha and -beta) to re...
Repository :
Europe PubMed Central
Language(s) :
English
Dynamic telomerase gene suppression via network effects of GSK3 inhibition
Author(s) :
Bilsland, A.E.
,
Hoare, S.F.
,
Stevenson, K.H.
,
Plumb, J.A.
,
Gomez-Roman, N.
,
Cairney, C.J.
,
Burns, S.
,
Lafferty-Whyte, K.
Description :
Background: Telomerase controls telomere homeostasis and cell immortality and is a promising anti-cancer target, but few small molecule telomerase inhibitors have been developed. Reactivated transcription of the catalytic subunit hTERT in cancer cells controls telomerase expression. Better un...
Repository :
Glasgow ePrints Service
Language(s) :
Undetermined
A mutation in a functional Sp1 binding site of the telomerase RNA gene (hTERC) promoter in a patient with Paroxysmal Nocturnal Haemoglobinuria
Author(s) :
Keith, W.N.
,
Vulliamy, T.
,
Zhao, J.
,
Ar, C.
,
Erzik, C.
,
Bilsland, A.
,
Ulku, B.
,
Marrone, A.
,
Mason, P.J.
,
Bessler, M.
,
Serakinci, N.
Description :
Background
Mutations in the gene coding for the RNA component of telomerase, hTERC, have been found in autosomal dominant dyskeratosis congenita (DC) and aplastic anemia. Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal blood disorder associated with aplastic anemia and characterized...
Mutations in the gene coding for the RNA component of telomerase, hTERC, have been found in autosomal dominant dyskeratosis congenita (DC) and aplastic anemia. Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal blood disorder associated with aplastic anemia and characterized...
Repository :
Glasgow ePrints Service
Language(s) :
Undetermined
Predictive value of thymidylate synthase and dihydropyrimidine dehydrogenase protein expression on survival in adjuvantly treated stage III colon cancer patients
Author(s) :
Westra, J.L.
,
Hollema, H
,
Schaapveld, M.
,
Platteel, I.
,
Oien, K.A.
,
Keith, W.N.
,
Mauritz, R.
,
Peters, G.J.
,
Buys, C.H.
Description :
BACKGROUND: The predictive value of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) expression on long-term survival by influencing 5-fluorouracil (5-FU) effect were determined in primary tumours and node metastases of stage III colon cancer patients treated adjuvantly with 5-FU ...
Repository :
DSpace at Vrije Universiteit Amsterdam
Language(s) :
Undetermined