Results
author = "Falkner, Falko G."
Tissue specificity of the initiation of immunoglobulin k gene transcription
Description :
The transient transcription of a rearranged mouse immunoglobulin k gene was studied in a monkey fibroblast cell line. The gene was inserted into an SV40 expression vector and the calcium phosphate coprecipitation method was used for transfection. The transcripts were correctly spliced; transcription...
Repository :
PUB - Publications at Bielefeld University
Language(s) :
English
Quantitation of Viral DNA by Real-Time PCR Applying Duplex Amplification, Internal Standardization, and Two-Color Fluorescence Detection
Description :
A real-time PCR method was developed to quantitate viral DNA that includes duplex amplification, internal standardization, and two-color fluorescence detection without the need to generate an external standardization curve. Applied to human parvovirus B19 DNA, the linear range was from 102 to at lea...
Repository :
Europe PubMed Central
Language(s) :
English
Generation of Transduction-Competent Retroviral Vectors by Infection with a Single Hybrid Vaccinia Virus
Author(s) :
Konetschny, Christian
,
Holzer, Georg W.
,
Urban, Carsten
,
Hämmerle, Thomas
,
Mayrhofer, Josef
,
Falkner, Falko G.
Description :
Recombinant vaccinia viruses that express defective retroviral vectors upon a single infection event in normal host cells were constructed. The gag-pol and envelope genes and a retroviral vector unit were inserted as vaccinia virus promoter-controlled transcription units at three separate loci. The ...
Repository :
Europe PubMed Central
Language(s) :
English
Selection of recombinant MVA by rescue of the essential D4R gene
Description :
Abstract
Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an enginee...
Repository :
DOAJ-Articles
Language(s) :
English
Pre-Clinical Efficacy and Safety of Experimental Vaccines Based on Non-Replicating Vaccinia Vectors against Yellow Fever
Author(s) :
Schäfer, Birgit
,
Holzer, Georg W.
,
Joachimsthaler, Alexandra
,
Coulibaly, Sogue
,
Schwendinger, Michael
,
Crowe, Brian A.
Description :
Repository :
Europe PubMed Central
Language(s) :
English
Selection of recombinant MVA by rescue of the essential D4R gene
Author(s) :
Ricci, Patricia S
,
Schäfer, Birgit
,
Kreil, Thomas R
,
Falkner, Falko G
,
Holzer, Georg W
Description :
Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant...
Repository :
Europe PubMed Central
Language(s) :
English
Binding of Murine Leukemia Virus Gag Polyproteins to KIF4, a Microtubule-Based Motor Protein
Author(s) :
Kim, Wankee
,
Tang, Yao
,
Okada, Yasushi
,
Torrey, Ted A.
,
Chattopadhyay, Sisir K.
,
Pfleiderer, Michael
,
Falkner, Falko G.
Description :
A cDNA clone encoding a cellular protein that interacts with murine leukemia virus (MuLV) Gag proteins was isolated from a T-cell lymphoma library. The sequence of the clone is identical to the C terminus of a cellular protein, KIF4, a microtubule-associated motor protein that belongs to the kinesin...
Repository :
Europe PubMed Central
Language(s) :
English
Nonreplicating Vaccinia Virus Vectors Expressing the H5 Influenza Virus Hemagglutinin Produced in Modified Vero Cells Induce Robust Protection▿
Author(s) :
Mayrhofer, Josef
,
Coulibaly, Sogue
,
Hessel, Annett
,
Holzer, Georg W.
,
Schwendinger, Michael
,
Brühl, Peter
,
Gerencer, Marijan
Description :
The timely development of safe and effective vaccines against avian influenza virus of the H5N1 subtype will be of the utmost importance in the event of a pandemic. Our aim was first to develop a safe live vaccine which induces both humoral and cell-mediated immune responses against human H5N1 influ...
Repository :
Europe PubMed Central
Language(s) :
English
A Pandemic Influenza H1N1 Live Vaccine Based on Modified Vaccinia Ankara Is Highly Immunogenic and Protects Mice in Active and Passive Immunizations
Author(s) :
Hessel, Annett
,
Schwendinger, Michael
,
Fritz, Daniela
,
Coulibaly, Sogue
,
Holzer, Georg W.
,
Sabarth, Nicolas
,
Kistner, Otfried
Description :
Repository :
Europe PubMed Central
Language(s) :
English
Vectors Based on Modified Vaccinia Ankara Expressing Influenza H5N1 Hemagglutinin Induce Substantial Cross-Clade Protective Immunity
Author(s) :
Hessel, Annett
,
Schwendinger, Michael
,
Holzer, Georg W.
,
Orlinger, Klaus K.
,
Coulibaly, Sogue
,
Savidis-Dacho, Helga
Description :
Repository :
Europe PubMed Central
Language(s) :
English