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author = "Ebright, Y W"
1

Found 7 documents, displaying page 1 of 1

Consensus DNA site for the Escherichia coli catabolite gene activator protein (CAP): CAP exhibits a 450-fold higher affinity for the consensus DNA site than for the E. coli lac DNA site.

Author(s) : Ebright, R H , Ebright, Y W , Gunasekera, A
Description : We have synthesized two 40 base pair DNA fragments; one fragment contains the consensus DNA site for CAP (fragment 'ICAP'); the other fragment contains the E. coli lac promoter DNA site for CAP (fragment 'LCAP'). We have investigated the binding of CAP to the two DNA fragments using the nitrocellulo...
Repository : Europe PubMed Central
Language(s) : English

Conversion of a helix-turn-helix motif sequence-specific DNA binding protein into a site-specific DNA cleavage agent.

Author(s) : Ebright, R H , Ebright, Y W , Pendergrast, P S , Gunasekera, A
Description : Escherichia coli catabolite gene activator protein (CAP) is a helix-turn-helix motif sequence-specific DNA binding protein [de Crombrugghe, B., Busby, S. & Buc, H. (1984) Science 224, 831-838; and Pabo, C. & Sauer, R. (1984) Annu. Rev. Biochem. 53, 293-321]. In this work, CAP has been converted into...
Repository : Europe PubMed Central
Language(s) : English

DNA-sequence recognition by CAP: role of the adenine N6 atom of base pair 6 of the DNA site.

Author(s) : Gunasekera, A , Ebright, Y W , Ebright, R H
Description : Two similar, but not identical, models have been proposed for the amino acid-base pair contacts in the CAP-DNA complex ('Model I,' Weber, I. and Steitz, T., Proc. Natl. Acad. Sci. USA, 81, 3973-3977, 1984; 'Model II,' Ebright, et al., Proc. Natl. Acad. Sci. USA, 81, 7274-7278, 1984). One difference ...
Repository : Europe PubMed Central
Language(s) : English

Determination of the orientation of a DNA binding motif in a protein-DNA complex by photocrosslinking.

Author(s) : Pendergrast, P S , Chen, Y , Ebright, Y W , Ebright, R H
Description : We have developed a straightforward biochemical method to determine the orientation of the DNA binding motif of a sequence-specific DNA binding protein relative to the DNA site in the protein-DNA complex. The method involves incorporation of a photoactivatable crosslinking agent at a single site wit...
Repository : Europe PubMed Central
Language(s) : English

High-resolution mapping of nucleoprotein complexes by site-specific protein-DNA photocrosslinking: organization of the human TBP-TFIIA-TFIIB-DNA quaternary complex.

Author(s) : Lagrange, T , Kim, T K , Orphanides, G , Ebright, Y W , Ebright, R H , Reinberg, D
Description : We have used a novel site-specific protein-DNA photocrosslinking procedure to define the positions of polypeptide chains relative to promoter DNA in binary, ternary, and quaternary complexes containing human TATA-binding protein, human or yeast transcription factor IIA (TFIIA), human transcription f...
Repository : Europe PubMed Central
Language(s) : English

Identification of amino acid-base contacts in the Myc-DNA complex by site-specific bromouracil mediated photocrosslinking.

Author(s) : Dong, Q , Blatter, E E , Ebright, Y W , Bister, K , Ebright, R H
Description : Myc binds to a 6 bp 2-fold symmetric DNA site: 5'-C-3A-2C-1G+1T+2G+3-3'. Using site-specific 5-bromouracil mediated photocrosslinking, we show that His336 of Myc contacts, or is close to, the thymine 5-methyl group at 2-fold symmetry-related positions -2 and +2 of the DNA site in the Myc-DNA complex...
Repository : Europe PubMed Central
Language(s) : English

DNA affinity cleaving analysis of homeodomain-DNA interaction: identification of homeodomain consensus sites in genomic DNA.

Author(s) : Shang, Z , Ebright, Y W , Iler, N , Pendergrast, P S , Echelard, Y , McMahon, A P , Ebright, R H , Abate, C
Description : We have incorporated the DNA-cleaving moiety o-phenanthroline-copper at amino acid 10 of the Msx-1 homeodomain, and we have analyzed site-specific DNA cleavage by the resulting Msx-1 derivative. We show that amino acid 10 of the Msx-1 homeodomain is close to the 5' end of the consensus DNA site 5'-(...
Repository : Europe PubMed Central
Language(s) : English
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Found 7 documents, displaying page 1 of 1