Results
author = "Chambon, P"
Sequence-specific activation of transcription by adenovirus EIa products is observed in HeLa cells but not in 293 cells.
Description :
The adenovirus EIa gene products activate transcription from the viral EIII and EIIaE promoters. We studied the mechanism of this stimulation by constructing a series of chimeric promoter recombinants containing the upstream regions of the EIII and EIIaE promoters linked to the TATA box-start-site r...
Repository :
Europe PubMed Central
Language(s) :
English
A rapid and efficient method for region- and strand-specific mutagenesis of cloned DNA.
Description :
The single-stranded viral DNA of an M13 phage recombinant containing the early promoter region of SV40 was hybridized with linear, double-stranded replicative form DNA of a related M13 phage containing a short deletion in the cloned SV40 sequence. The heteroduplexes formed between these DNA molecule...
Repository :
Europe PubMed Central
Language(s) :
English
Purification of mRNA guanylyltransferase from calf thymus.
Description :
mRNA guanylyltransferase has been extensively purified from calf thymus. A GTP-binding assay was used based on the observations by Shuman and Hurwitz (1981) and Venkatesan and Moss (1982) that vaccinia virus and HeLa cell mRNA guanylyltransferases bind the GMP moiety from GTP in the absence of an ac...
Repository :
Europe PubMed Central
Language(s) :
English
The mouse immunoglobulin heavy-chain enhancer: effect on transcription in vitro and binding of proteins present in HeLa and lymphoid B cell extracts.
Description :
The ability of the mouse immunoglobulin heavy chain gene (IgH) enhancer to stimulate in vitro transcription from the adenovirus-2 major late promoter (Ad2MLP) has been investigated. In agreement with the in vivo cell-type specificity of this enhancer, a stimulation can be observed in BJA-B lymphoid ...
Repository :
Europe PubMed Central
Language(s) :
English
A single-stranded DNA-binding protein promotes the binding of the purified oestrogen receptor to its responsive element.
Description :
The purified human oestrogen receptor (hER) does not form a detectable complex with an oestrogen responsive element (ERE) under conditions where hER-ERE complexes are readily formed with crude extracts from Hela or yeast cells expressing the hER. This indicates that other factor(s) are necessary for...
Repository :
Europe PubMed Central
Language(s) :
English
DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes.
Description :
The restriction enzymes HhaI and HpaII, whose activity is inhibited by cytosine methylation within their recognition sites, have been utilised as probes to study methylation in the vicinity of the ovalbumin gene in DNA from various chicken tissues. This was complemented by a preliminary study of met...
Repository :
Europe PubMed Central
Language(s) :
English
Deletions covering the putative promoter region of early mRNAs of simian virus 40 do not abolish T-antigen expression.
Description :
A recombinant plasmid was constructed by insertion of the early genes of simian virus 40 (SV40) into pBR322. When it was introduced into eukaryotic cells, the SV40 early genes were expressed. We have made deletion mutants of this plasmid, from which the major cap sites of SV40 early mRNAs have been ...
Repository :
Europe PubMed Central
Language(s) :
English
RAR-beta 4, a retinoic acid receptor isoform is generated from RAR-beta 2 by alternative splicing and usage of a CUG initiator codon.
Description :
An isoform of retinoic acid receptor beta, RAR-beta 4, has been identified. RAR-beta 4 is expressed under the control of the same retinoic acid-responsive promoter as RAR-beta 2. RAR-beta 4, which is generated by alternative splicing from the same primary transcripts as RAR-beta 2, is initiated by a...
Repository :
Europe PubMed Central
Language(s) :
English
A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.
Description :
We have previously shown that a T to G transversion at the second T of the conalbumin "TATA" box drastically decreases specific initiation of transcription by RNA polymerase B (1). We now report that a T to A transcription at the same nucleotide also drastically decreases transcription, suggesting t...
Repository :
Europe PubMed Central
Language(s) :
English
F9 embryocarcinoma cells, a cell autonomous model to study the functional selectivity of RARs and RXRs in retinoid signaling
Description :
Mouse F9 embryocarcinoma (EC) cells
constitute a well established cell-autonomous model
system for investigating retinoid signaling in vitro as,
depending on culture conditions, retinoic acid (RA) can
induce their differentiation into either primitive, parietal
or visceral extraembryonic endoderm-li...
Repository :
DIGITUM. Depósito Digital Institucional de la Universidad de Murcia.
Language(s) :
English