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author = "Chambon, P"

Found 198 documents, displaying page 3 of 20

Short and long range activation by the SV40 enhancer.

Author(s) : Wasylyk, B , Wasylyk, C , Chambon, P
Description : Activation of transcription by the SV40 enhancer decreases in an apparently biphasic manner when DNA sequences are interposed between the SV40 enhancer and either the homologous SV40 early or the heterologous conalbumin promoter elements. With increasing lengths of short DNA fragments (up to about 1...
Repository : Europe PubMed Central
Language(s) : English

Clustering of RNA polymerase B molecules in the 5' moiety of the adult beta-globin gene of hen erythrocytes.

Description : Nuclei were prepared from mature and immature hen erythrocytes and incubated for RNA synthesis in the absence or in the presence of Sarkosyl. The in vitro labelled synthesized RNA was hybridized to specific 5' and 3' fragments of the chicken adult beta-globin gene to investigate the possible presenc...
Repository : Europe PubMed Central
Language(s) : English

DNA methylation: correlation with DNase I sensitivity of chicken ovalbumin and conalbumin chromatin.

Author(s) : Kuo, M T , Mandel, J L , Chambon, P
Description : To analyse the relationship between DNA undermethylation at some sites in the ovalbumin and conalbumin gene regions (1) and the expression of these genes in chick oviduct, digestions with HhaI, which differentiates between methylated and unmethylated HhaI restriction sites, was performed on DNA isol...
Repository : Europe PubMed Central
Language(s) : English

Corrected splicing of a chicken ovalbumin gene transcript in mouse L cells.

Description : Mouse thymidine-kinase-negative L cells were transformed with a cloned chicken ovalbumin gene linked to the cloned thymidine kinase gene from herpes simplex virus type 1. Most thymidine-kinase-positive clones contained one or more copies of the ovalbumin gene, which were stably maintained for at lea...
Repository : Europe PubMed Central
Language(s) : English

Mouse retinoic acid receptor alpha 2 isoform is transcribed from a promoter that contains a retinoic acid response element.

Author(s) : Leroy, P , Nakshatri, H , Chambon, P
Description : We have characterized the promoter of the mouse retinoic acid receptor alpha 2 (mRAR-alpha 2) isoform. This promoter contains a retinoic acid response element (RARE) that closely resembles the RARE that is present in the RAR-beta 2 promoter. Moreover, RAR-alpha 2 and RAR-beta 2 proximal promoter seq...
Repository : Europe PubMed Central
Language(s) : English

Developmental abnormalities in cultured mouse embryos deprived of retinoic by inhibition of yolk-sac retinol binding protein synthesis.

Author(s) : Båvik, C , Ward, S J , Chambon, P
Description : Presomitic and 3- to 12-somite pair cultured mouse embryos were deprived of retinoic acid (RA) by yolk-sac injections of antisense oligodeoxynucleotides for retinol binding protein (RBP). Inhibition of yolk-sac RBP synthesis was verified by immunohistochemistry, and the loss of activity of a lacZ-co...
Repository : Europe PubMed Central
Language(s) : English

The repeated GC-rich motifs upstream from the TATA box are important elements of the SV40 early promoter.

Author(s) : Everett, R D , Baty, D , Chambon, P
Description : We have constructed deletion and point mutations within the Simian virus 40 (SV40) early promoter region which contains two tandemly repeated 21 bp sequences and a related 22 bp sequence (the "upstream" 21 bp repeat region). After transfection into permissive CV-1 cells and non-permissive mouse 3T3-...
Repository : Europe PubMed Central
Language(s) : English

Preferential in vitro assembly of nucleosome cores on some AT-rich regions of SV40 DNA.

Author(s) : Wasylyk, B , Oudet, P , Chambon, P
Description : We have found that nucleosomes reconstituted from histone octamers and SV40 DNA Form I by progressively decreasing the salt concentration from 2 M NaCl are formed preferentially around 0.27, 0.37, 0.50 and 0.85 on SV40 DNA (relative to the EcoRI site). When SV40 DNA Form III is used, the nucleosomes...
Repository : Europe PubMed Central
Language(s) : English

Adenovirus DNA template for late transcription is not a replicative intermediate.

Description : The relationship between adenovirus replication and late transcription has been investigated using viral replication and transcription complexes isolated from infected HeLa cell nuclei. These two types of complexes extracted from adenovirus type 2-infected cell nuclei did not sediment at the same ra...
Repository : Europe PubMed Central
Language(s) : English

The same amount of DNA is organized in in vitro-assembled nucleosomes irrespective of the origin of the histones.

Author(s) : Spadafora, C , Oudet, P , Chambon, P
Description : The four histones H2A, H2B, H3 and H4 from calf thymus, CHO and sea urchin gastrula cells were associated by stepwise dialysis from 2 M NaCl with SV40 DNA Form I. The in vitro-assembled chromatins were visualized by electron microscopy and the size of the DNA fragments generated by digestion with DN...
Repository : Europe PubMed Central
Language(s) : English

Found 198 documents, displaying page 3 of 20