Results
author = "Blumenfeld, M"
Drosophila melanogaster H1 histone is phosphorylated stably.
Description :
Phosphorylation of histone H1 is developmentally regulated in Drosophila spp. It cannot be detected in preblastoderm embryos or polytene salivary gland cells, but in cellular blastoderm, postblastoderm embryo, and amitotic adult head nuclei, it occurs with a frequency of roughly 4 x 10(5) molecules ...
Repository :
Europe PubMed Central
Language(s) :
English
Nucleotide sequence of a Drosophila melanogaster H1 histone gene.
Description :
Repository :
Europe PubMed Central
Language(s) :
English
Drosophila nucleosomes contain an unusual histone-like protein.
Description :
Mononucleosomes prepared from Drosophila melanogaster nuclei contain the four core histones H2A, H2B, H3, and H4 plus an additional histone-like, acid-soluble, chromosomal protein. It is probably the protein designated D2 by Alfageme et al. [Alfageme, C.R., Zweidler, A., Mahowald, A. & Cohen, L.H. (...
Repository :
Europe PubMed Central
Language(s) :
English
Ex vivo regulation of specific gene expression by nanomolar concentration of double-stranded dumbbell oligonucleotides.
Description :
Inhibition of specific transcriptional regulatory proteins is a new approach to control gene expression. Transcriptional activity of DNA-binding proteins can be inhibited by the use of double-stranded (ds) oligodeoxynucleotides that compete for the binding to their specific target sequences in promo...
Repository :
Europe PubMed Central
Language(s) :
English
Self-cleavage of a 71 nucleotide-long ribozyme derived from hepatitis delta virus genomic RNA.
Description :
Self-cleavage efficiency of ribozymes derived from hepatitis delta virus (HDV) has been shown to depend on the RNA structure, which in turn may be determined by the length of the considered sequences. Here we describe the construction and functional analysis of a 71 nucleotide-long RNA genomic fragm...
Repository :
Europe PubMed Central
Language(s) :
English
A distal dimerization domain is essential for DNA-binding by the atypical HNF1 homeodomain.
Description :
Hepatic Nuclear Factor 1 (HNF1, also referred to as LFB1, HP1 or APF) is a liver-specific transcription factor required for the expression of many hepatocyte specific genes. We report here the purification of this rat liver nuclear protein and the cloning of its cDNA using a PCR-derived approach. Se...
Repository :
Europe PubMed Central
Language(s) :
English
Crosslinking of double-stranded oligonucleotides containing O-methyl-substituted pyrophosphate groups to the HNF1 transcription factor in nuclear cell extract.
Author(s) :
Kuznetsova, S A
,
Clusel, C
,
Ugarte, E
,
Elias, I
,
Vasseur, M
,
Blumenfeld, M
,
Shabarova, Z A
Description :
Probing of the HNF1 (hepatocyte nuclear factor I) DNA-binding region using a set of DNA duplexes containing pyrophosphate or O-methyl-substituted pyrophosphate internucleotide groups at different positions of the HNF1 recognition sequence was performed. The histidine-tagged HNF1/1-281 DNA binding do...
Repository :
Europe PubMed Central
Language(s) :
English
Drosophila histone H2A.2 is associated with the interbands of polytene chromosomes.
Description :
Drosophila chromatin contains two antigenically distinct H2A histones, H2A.1 and H2A.2. Indirect immunofluorescence analyses revealed that anti-H2A.1 binding was distributed throughout polytene chromosomes, whereas anti-H2A.2 binding was interband-specific. Thus, H2A.2 probably contributes to the le...
Repository :
Europe PubMed Central
Language(s) :
English
Oligonucleotide circularization by template-directed chemical ligation.
Author(s) :
Dolinnaya, N G
,
Blumenfeld, M
,
Merenkova, I N
,
Oretskaya, T S
,
Krynetskaya, N F
,
Ivanovskaya, M G
,
Vasseur, M
,
Shabarova, Z A
Description :
An efficient method for producing the covalent closure of oligonucleotides on complementary templates by the action of BrCN was developed. A rational design of linear precursor oligonucleotides was studied, and the effect of factors such as oligonucleotide concentration and oligomer-template length ...
Repository :
Europe PubMed Central
Language(s) :
English
Anomalous electrophoretic mobility of Drosophila phosphorylated H1 histone: is it related to the compaction of satellite DNA into heterochromatin?
Description :
In embryonic nuclei of Drosophila virilis, 45% of the DNA is satellite, and congruent to 50% of the H1 histone is phosphorylated. In polytene salivary gland nuclei, less than 1% of the DNA is satellite, and less than 10tion. The phosphorylated H1's migrate 4% slower than the unphosphorylated H1's ...
Repository :
Europe PubMed Central
Language(s) :
English