Results
author = "Aladjem, Mirit I"
Initiation of DNA replication at the human β-globin 3′ enhancer
Description :
The origin of DNA replication in the human β-globin gene contains an initiation region (IR) and two flanking auxiliary elements. Two replicator modules are located within the upstream auxiliary sequence and the IR core, but the functional sequences in the downstream auxiliary element are unknown. He...
Repository :
Europe PubMed Central
Language(s) :
English
The Human β-Globin Replication Initiation Region Consists of Two Modular Independent Replicators
Author(s) :
Wang, Lixin
,
Lin, Chii-Mei
,
Brooks, Sarah
,
Cimbora, Dan
,
Groudine, Mark
,
Aladjem, Mirit I.
Description :
Previous studies have shown that mammalian cells contain replicator sequences, which can determine where DNA replication initiates. However, the specific sequences that confer replicator activity were not identified. Here we report a detailed analysis of replicator sequences that dictate initiation ...
Repository :
Europe PubMed Central
Language(s) :
English
A formal MIM specification and tools for the common exchange of MIM diagrams: an XML-Based format, an API, and a validation method
Author(s) :
Luna, Augustin
,
Karac, Evrim I
,
Sunshine, Margot
,
Chang, Lucas
,
Nussinov, Ruth
,
Aladjem, Mirit I
,
Kohn, Kurt W
Description :
Repository :
Europe PubMed Central
Language(s) :
English
Prevention of Transcriptional Silencing by a Replicator-Binding Complex Consisting of SWI/SNF, MeCP1, and hnRNP C1/C2 ▿
Author(s) :
Huang, Liang
,
Fu, Haiqing
,
Lin, Chii Mei
,
Conner, Amy L.
,
Zhang, Ya
,
Aladjem, Mirit I.
Description :
Transcriptional silencing selectively impedes gene expression. Silencing is often accompanied by replication delay and can be prevented by replicator sequences. Here we report a replicator-binding protein complex involved in the prevention of transcriptional silencing. The protein complex interacts ...
Repository :
Europe PubMed Central
Language(s) :
English
PathVisio-MIM: PathVisio plugin for creating and editing Molecular Interaction Maps (MIMs)
Author(s) :
Luna, Augustin
,
Sunshine, Margot L.
,
van Iersel, Martijn P.
,
Aladjem, Mirit I.
,
Kohn, Kurt W.
Description :
Motivation: A plugin for the Java-based PathVisio pathway editor has been developed to help users draw diagrams of bioregulatory networks according to the Molecular Interaction Map (MIM) notation. Together with the core PathVisio application, this plugin presents a simple to use and cross-platform a...
Repository :
Europe PubMed Central
Language(s) :
English
PathVisio-Validator: a rule-based validation plugin for graphical pathway notations
Author(s) :
Chandan, Kumar
,
van Iersel, Martijn P.
,
Aladjem, Mirit I.
,
Kohn, Kurt W.
,
Luna, Augustin
Description :
Purpose: The PathVisio-Validator plugin aims to simplify the task of producing biological pathway diagrams that follow graphical standardized notations, such as Molecular Interaction Maps or the Systems Biology Graphical Notation. This plugin assists in the creation of pathway diagrams by ensuring c...
Repository :
Europe PubMed Central
Language(s) :
English
DNA methylation supports intrinsic epigenetic memory in mammalian cells.
Author(s) :
Feng Yong-Qing
,
Desprat Romain
,
Fu Haiqing
,
Olivier Emmanuel
,
Lin Chii Mei
,
Lobell Amanda
,
Gowda Shilpa N
,
Aladjem Mirit I
Description :
We have investigated the role of DNA methylation in the initiation and maintenance of silenced chromatin in somatic mammalian cells. We found that a mutated transgene, in which all the CpG dinucleotides have been eliminated, underwent transcriptional silencing to the same extent as the unmodified tr...
Repository :
DOAJ-Articles
Language(s) :
Undetermined
DNA Methylation Supports Intrinsic Epigenetic Memory in Mammalian Cells
Author(s) :
Feng, Yong-Qing
,
Desprat, Romain
,
Fu, Haiqing
,
Olivier, Emmanuel
,
Lin, Chii Mei
,
Lobell, Amanda
,
Gowda, Shilpa N
Description :
We have investigated the role of DNA methylation in the initiation and maintenance of silenced chromatin in somatic mammalian cells. We found that a mutated transgene, in which all the CpG dinucleotides have been eliminated, underwent transcriptional silencing to the same extent as the unmodified tr...
Repository :
Europe PubMed Central
Language(s) :
English
Replication Initiation Patterns in the β-Globin Loci of Totipotent and Differentiated Murine Cells: Evidence for Multiple Initiation Regions
Author(s) :
Aladjem, Mirit I.
,
Rodewald, Luo Wei
,
Lin, Chii Mai
,
Bowman, Sarah
,
Cimbora, Daniel M.
,
Brody, Linnea L.
,
Epner, Elliot M.
Description :
The replication initiation pattern of the murine β-globin locus was analyzed in totipotent embryonic stem cells and in differentiated cell lines. Initiation events in the murine β-globin locus were detected in a region extending from the embryonic Ey gene to the adult βminor gene, unlike the restric...
Repository :
Europe PubMed Central
Language(s) :
English
The Human β-Globin Locus Control Region Can Silence as Well as Activate Gene Expression
Author(s) :
Feng, Yong-Qing
,
Warin, Renaud
,
Li, Taihao
,
Olivier, Emmanuel
,
Besse, Arnaud
,
Lobell, Amanda
,
Fu, Haiqing
,
Lin, Chii Mei
Description :
Using recombinase-mediated cassette exchange to test multiple transgenes at the same site of integration, we demonstrate a novel chromatin context-dependent silencer activity of the β-globin locus control region (LCR). This silencer activity requires DNase I hypersensitive sites HS2 and HS3 but not ...
Repository :
Europe PubMed Central
Language(s) :
English