Results
author = "Webster, R G"
Interplay between carbohydrate in the stalk and the length of the connecting peptide determines the cleavability of influenza virus hemagglutinin.
Description :
The ability of many viruses to replicate in host cells depends on cleavage of certain viral glycoproteins, including hemagglutinin (HA). By generating site-specific mutant HAs of two highly virulent influenza viruses, we established that the relationship between carbohydrate in the stalk and the len...
Repository :
Europe PubMed Central
Language(s) :
English
Defective interfering virus associated with A/Chicken/Pennsylvania/83 influenza virus.
Description :
The A/Chicken/Pennsylvania/1/83 influenza virus, isolated from a respiratory infection of chickens, is an avirulent H5N2 virus containing subgenomic RNAs (W.J. Bean, Y. Kawaoka, J.M. Wood, J.E. Pearson, and R.G. Webster, J. Virol. 54:151-160, 1985). We show here that defective interfering particles ...
Repository :
Europe PubMed Central
Language(s) :
English
Influenza: an emerging disease.
Author(s) :
Webster, R. G.
Description :
Because all known influenza A subtypes exist in the aquatic bird reservoir, influenza is not an eradicable disease; prevention and control are the only realistic goals. If people, pigs, and aquatic birds are the principal variables associated with interspecies transfer of influenza virus and the eme...
Repository :
Europe PubMed Central
Language(s) :
English
Sequence requirements for cleavage activation of influenza virus hemagglutinin expressed in mammalian cells.
Description :
Cleavage of the hemagglutinin (HA) in tissue culture systems has been correlated with virulence of avian influenza viruses. To examine the structural requirements for cleavage of the HA, the HA gene from a virulent H5 influenza virus was expressed in mammalian cells (CV-1), and the cleavage site of ...
Repository :
Europe PubMed Central
Language(s) :
English
Matrix protein from influenza A virus and its role in cross-protection in mice.
Description :
The matrix (M) protein of influenza A virus, one of the two group-specific internal proteins of the virion, was isolated in a pure form, and its immunogenicity was stable to heating at 100 degrees C for 2 min. Mice immunized with isolated M protein in complete Freund adjuvant and subsequently infect...
Repository :
Europe PubMed Central
Language(s) :
English
Steps in maturation of influenza A virus neuraminidase.
Description :
We have studied the maturation of the influenza A virus neuraminidase (NA), using monoclonal antibodies (MAbs) with different conformational specificities against the head domains of the N8 NA. The results obtained with radioimmunoprecipitation, together with previously published information, sugges...
Repository :
Europe PubMed Central
Language(s) :
English
Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics.
Description :
We determined the origin and evolutionary pathways of the PB1 genes of influenza A viruses responsible for the 1957 and 1968 human pandemics and obtained information on the variable or conserved region of the PB1 protein. The evolutionary tree constructed from nucleotide sequences suggested the foll...
Repository :
Europe PubMed Central
Language(s) :
English
Impairment of multicycle influenza virus growth in Vero (WHO) cells by loss of trypsin activity.
Description :
We demonstrated that influenza virus replication in Vero (WHO) cells, a subline of African green monkey kidney cells, is impaired by rapid inactivation of trypsin in the culture fluids. Trypsin inactivation was caused by a factor secreted by Vero cells into the media. Repeated addition of trypsin to...
Repository :
Europe PubMed Central
Language(s) :
English