Results
author = "Olive, P."
Response of spheroids implanted in the peritoneal cavity of mice exposed to cyclophosphamide and ionizing radiation.
Author(s) :
Olive, P. L.
Description :
Chinese hamster V79-171B spheroids implanted in the peritoneal cavity of C3H mice were characterized as a model for evaluating the toxicity of drugs requiring metabolic activation in vivo. After 24 hours in the peritoneal cavity, spheroid cellularity and plating efficiency were not significantly dec...
Repository :
Europe PubMed Central
Language(s) :
English
Hydroethidine: a fluorescent redox probe for locating hypoxic cells in spheroids and murine tumours.
Author(s) :
Olive, P. L.
Description :
The fluorescent redox probe hydroethidine was accumulated and metabolised about five times faster in aerobic than in hypoxic mammalian cells. Patterns of fluorescence in Chinese hamster V79 spheroids also indicated that internal hypoxic cells were less able to metabolise the drug; toxicity was obser...
Repository :
Europe PubMed Central
Language(s) :
English
Detection of hypoxia by measurement of DNA damage in individual cells from spheroids and murine tumours exposed to bioreductive drugs. II. RSU 1069.
Author(s) :
Olive, P. L.
Description :
The ability of the dual-function bioreductive drug, RSU 1069, to identify hypoxic cells in multicell spheroids and murine SCCVII squamous cell carcinomas was examined using the alkaline comet method. This method applies fluorescence microscopy and image analysis to measure the amount of migration of...
Repository :
Europe PubMed Central
Language(s) :
English
Detection of hypoxia by measurement of DNA damage in individual cells from spheroids and murine tumours exposed to bioreductive drugs. I. Tirapazamine.
Author(s) :
Olive, P. L.
Description :
The possibility of using tirapazamine (SR 4233) to identify hypoxic cells in multicell spheroids and murine tumours was examined by measuring tirapazamine-induced DNA damage to individual cells from multicell spheroids and SCCVII murine tumours. Fluorescence microscopy and image analysis were used t...
Repository :
Europe PubMed Central
Language(s) :
English
Different sensitivity to cytotoxic agents of internal and external cells of spheroids composed of thioguanine-resistant and sensitive cells.
Author(s) :
Olive, P. L.
Description :
When thioguanine-resistant V79 cells are introduced into spinner flasks containing V79 multicell spheroids, the mutant cells attach to the surface of the spheroids. The composite spheroids thus formed consist of external, thioguanine-resistant (TGr) and internal, thioguanine-sensitive cells. Cell so...
Repository :
Europe PubMed Central
Language(s) :
English
Inhibition of DNA synthesis by nitroheterocycles. II. Mechanisms of cytotoxicity.
Author(s) :
Olive, P. L.
Description :
Nitroheterocycles have been shown to inhibit the incorporation of 3H-TdR by cultured L-929 cells, and the degree of inhibition is related to their electron-affinity. On the basis of their chemical reactivity and potential clinical utility, nitrofurazone, misonidazole and metronidazole were selected ...
Repository :
Europe PubMed Central
Language(s) :
English
Inhibition of DNA synthesis by nitroheterocycles. I. Correlation with half-wave reduction potential.
Author(s) :
Olive, P. L.
Description :
Twenty-one nitroheterocycles, including metronidazole, misonidazole and AF-2, were tested for their ability to inhibit DNA synthesis in mouse L-929 cells growing in culture. All those tested inhibited the rate of incorporation of 3H-thymidine into L cells following drug treatment for 4 h under aerob...
Repository :
Europe PubMed Central
Language(s) :
English
Use of the comet assay for assessment of drug resistance and its modulation in vivo.
Description :
Drug resistance is generally considered to be a major impediment to successful cancer chemotherapy, yet it is generally not possible to predict the degree or timing of the emergence of tumour resistance in most chemotherapy protocols. Recent developments with the single-cell gel electrophoresis or '...
Repository :
Europe PubMed Central
Language(s) :
English
Radiosensitisation and radioprotection by BSO and WR-2721: the role of oxygenation.
Description :
Endogenous and exogenous thiols are thought to influence cellular radiosensitivity directly by radical scavenging and/or hydrogen donation processes, and indirectly, by regulating the amount of oxygen (or other electron affinic radiosensitiser) able to reach the radiosensitive targets of the cell. T...
Repository :
Europe PubMed Central
Language(s) :
English