Results
author = "Goodman, H M"
CpG methylation inhibits proenkephalin gene expression and binding of the transcription factor AP-2.
Description :
DNA methylation at HpaII (CmCGG) sites inhibits expression of a human proenkephalin-CAT fusion gene when it is transiently expressed in CV-1 cells or stably expressed in C6-glioma cells. The inhibitory effects of HpaII methylation have been mapped to a site within the human proenkephalin promoter lo...
Repository :
Europe PubMed Central
Language(s) :
English
Refractoriness to growth hormone is associated with increased intracellular calcium in rat adipocytes.
Description :
In adipocytes that have been deprived of growth hormone (GH) for at least 3 hr, GH elicits a transient insulin-like response that is followed by a period of refractoriness to further insulin-like stimulation. Exposure of adipocytes to GH in the first hour of a 3-hr incubation prevents the appearance...
Repository :
Europe PubMed Central
Language(s) :
English
In vitro transcription of RU, a middle repetitive element of the rat genome.
Description :
Repetitive DNA sequences have been found within as well as adjacent to the rat growth hormone (rGH) gene (1,2). In vitro transcription of the rGH gene yields transcripts predominantly from a tandemly duplicated repeat (RU) in the second intron of the gene. The relative alpha-amanitin sensitivity of ...
Repository :
Europe PubMed Central
Language(s) :
English
Isolation and characterization of cDNA clones encoding pathogenesis-related proteins from tobacco mosaic virus infected tobacco plants.
Description :
Infection of the tobacco cultivar Samsun NN by tobacco mosaic virus (TMV) results in a hypersensitive response. During this defense reaction several host encoded proteins, known as pathogenesis-related proteins (PR-proteins), are induced. Poly(A)+ RNA from TMV infected tobacco plants was used to con...
Repository :
Europe PubMed Central
Language(s) :
English
DNA sequence of the rat growth hormone gene: location of the 5' terminus of the growth hormone mRNA and identification of an internal transposon-like element.
Description :
The present communication describes the molecular cloning and DNA sequence determination of the rat growth hormone (rGH) gene. The rGH gene was cloned on an 11 kilobase EcoRI fragment of total rat DNA; it has four intervening sequences which correspond in position to those of the human growth hormon...
Repository :
Europe PubMed Central
Language(s) :
English
Comparative fluorescence in situ hybridization mapping of a 431-kb Arabidopsis thaliana bacterial artificial chromosome contig reveals the role of chromosomal duplications in the expansion of the Brassica rapa genome.
Description :
Comparative genome studies are important contributors to our understanding of genome evolution. Most comparative genome studies in plants have been based on genetic mapping of homologous DNA loci in different genomes. Large-scale comparative physical mapping has been hindered by the lack of efficien...
Repository :
Europe PubMed Central
Language(s) :
English
Molecular cloning of the avian myelocytomatosis virus genome and recovery of infectious virus by transfection of chicken cells.
Description :
The avian retrovirus myelocytomatosis virus 19 (MCV) possesses an interesting diversity of oncogenic potentials, but the virus has proven difficult to study because of its inability to replicate without the assistance of a helper virus. We have therefore isolated and amplified the genome of MCV by m...
Repository :
Europe PubMed Central
Language(s) :
English
Human growth hormone DNA sequence and mRNA structure: possible alternative splicing.
Description :
We have determined the complete sequence of the human growth hormone (hGH) gene and the position of the mature 5' end of the hGH mRNA within the sequence. Comparison of this sequence with that of a cloned hGH cDNA shows that the gene is interrupted by four intervening sequences. S1 mapping shows tha...
Repository :
Europe PubMed Central
Language(s) :
English
Identification of a plant serine-arginine-rich protein similar to the mammalian splicing factor SF2/ASF.
Description :
We show that the higher plant Arabidopsis thaliana has a serine-arginine-rich (SR) protein family whose members contain a phosphoepitope shared by the animal SR family of splicing factors. In addition, we report the cloning and characterization of a cDNA encoding a higher-plant SR protein from Arabi...
Repository :
Europe PubMed Central
Language(s) :
English
Sequences spanning the EcoRI substrate site.
Description :
Substrate recognition by the EcoRI restriction endonuclease was investigated by analysis of the nucleotide sequences at the sites of enzymatic cleavage in various DNA molecules. 5'-end labeling and homochromatographic fingerprinting led to the determination of a 17-base-pair sequence spanning the Ec...
Repository :
Europe PubMed Central
Language(s) :
English