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author = "Dehottay, Philippe"
1
Found 6 documents, displaying page 1 of 1
Cloning and amplified expression in streptomyces-lividans of the gene encoding the extracellular beta-lactamase from streptomyces-cacaoi
Author(s) :
Lenzini, Mauro V
,
Nojima, Shiego
,
Dusart, Jean
,
Ogawara, Hiroshi
,
Dehottay, Philippe
,
Frère, Jean-Marie
,
Ghuysen, Jean-Marie
Description :
A 19 kb SphI DNA fragment containing the gene for the extracellular active-site serine beta-lactamase of Streptomyces cacaoi KCC-SO352 was cloned in Streptomyces lividans TK24 using the high-copy-number plasmid pIJ702 as vector. A 30-fold higher yield of beta-lactamase was obtained from S. lividans ...
Repository :
ORBi (University of Liège)
Language(s) :
English
Cloning and amplified expression in Streptomyces lividans of a gene encoding extracellular beta-lactamase from Streptomyces albus G
Author(s) :
Dehottay, Philippe
,
Dusart, Jean
,
Duez, Colette
,
Lenzini, Mauro V.
,
Martial, Joseph
,
Frère, Jean-Marie
,
Ghuysen, Jean-Marie
Description :
A 4.9-kb DNA fragment containing the bla gene for the extracellular beta-lactamase (BLA) of Streptomyces albus G was cloned in Streptomyces lividans using the conjugative, low-copy-number plasmid pIJ61 as vector. No expression of bla was observed when this DNA fragment was introduced into Escherichi...
Repository :
ORBi (University of Liège)
Language(s) :
English
The crystal structure of the beta-lactamase of Streptomyces albus G at 0.3 nm resolution.
Author(s) :
Dideberg, Otto
,
Charlier, Paulette
,
Wery, Jean-Paul
,
Dehottay, Philippe
,
Dusart, Jean
,
Erpicum, Thomas
,
Frère, Jean-Marie
Description :
The crystal structure of the beta-lactamase of Streptomyces albus G has been solved at 0.3 nm resolution by X-ray-diffraction methods. The enzyme is a typical two-domain protein. One domain consists of five alpha-helices, and the other is five-stranded beta-sheet with alpha-helices on both sides of ...
Repository :
ORBi (University of Liège)
Language(s) :
English
Nucleotide sequence of the gene encoding the Streptomyces albus G beta-lactamase precursor
Author(s) :
Dehottay, Philippe
,
Dusart, Jean
,
De Meester, Fabien
,
Joris, Bernard
,
Van Beeumen, Jozef
,
Erpicum, Thomas
,
Frère, Jean-Marie
Description :
A 1400-base DNA fragment, which contains the gene encoding the extracellular active-site serine beta-lactamase of Streptomyces albus G previously cloned into Streptomyces lividans [Dehottay et al. (1986) Gene 42, 31-36], was sequenced. The gene codes for a 314-amino-acid precursor, the N-terminal re...
Repository :
ORBi (University of Liège)
Language(s) :
English
The active-sites of the beta-lactamases of the streptomyces-cacaoi and streptomyces-albus-G
Author(s) :
Demeester, Fabien
,
Joris, Bernard
,
Lenzini, Mauro V.
,
Dehottay, Philippe
,
Erpicium, Thomas
,
Dusart, Jean
,
Klein, Daniel
Description :
The active-site serine of the extracellular beta-lactamases of Streptomyces cacaoi and Streptomyces albus G has been labelled with beta-iodopenicillanate. The determination of the sequence of the labelled peptides obtained after trypsin digestion of the denatured proteins indicate both enzymes to be...
Repository :
ORBi (University of Liège)
Language(s) :
English
Cloning and amplified expression in Streptomyces lividans of a gene encoding extracellular beta-lactamase from Streptomyces albus G
Author(s) :
Dehottay, Philippe
,
Dusart, Jean
,
Duez, Colette
,
Lenzini, Mauro V.
,
Martial, Joseph
,
Frère, Jean-Marie
,
Ghuysen, Jean-Marie
Description :
A 4.9-kb DNA fragment containing the bla gene for the extracellular beta-lactamase (BLA) of Streptomyces albus G was cloned in Streptomyces lividans using the conjugative, low-copy-number plasmid pIJ61 as vector. No expression of bla was observed when this DNA fragment was introduced into Escherichi...
Repository :
ORBi (University of Liège)
Language(s) :
English
1
Found 6 documents, displaying page 1 of 1