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Cloning and amplified expression in Streptomyces lividans of a gene encoding extracellular beta-lactamase from Streptomyces albus G

Description : A 4.9-kb DNA fragment containing the bla gene for the extracellular beta-lactamase (BLA) of Streptomyces albus G was cloned in Streptomyces lividans using the conjugative, low-copy-number plasmid pIJ61 as vector. No expression of bla was observed when this DNA fragment was introduced into Escherichi...
Language(s) : English
Subject(s) : recombinant DNA , plasmids , dd-peptidases , branched pathway , ampicillin resistance , Actinomycetes , Life sciences :: Microbiology [F11] , Sciences du vivant :: Microbiologie [F11] , Physical, chemical, mathematical & earth Sciences :: Space science, astronomy & astrophysics [G05] , Physique, chimie, mathématiques & sciences de la terre :: Aérospatiale, astronomie & astrophysique [G05]
Publisher(s) :
Contributor(s) : Fonds de la Recherche Scientifique Médicale - FRSM
Source(s) : &ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&jtitle=Gene&issn=0378-1119&volume=42&pages=31-36&pub=Elsevier+Science&place=Amsterdam&place=The+Netherlands
Publication Date(s) : 1986-01-01