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• (author = "Dehottay, Philippe")
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Cloning and amplified expression in streptomyces-lividans of the gene encoding the extracellular beta-lactamase from streptomyces-cacaoi

Author(s) : Lenzini, Mauro V , Nojima, Shiego , Dusart, Jean , Ogawara, Hiroshi , Dehottay, Philippe , Frère, Jean-Marie , Ghuysen, Jean-Marie
Description : A 19 kb SphI DNA fragment containing the gene for the extracellular active-site serine beta-lactamase of Streptomyces cacaoi KCC-SO352 was cloned in Streptomyces lividans TK24 using the high-copy-number plasmid pIJ702 as vector. A 30-fold higher yield of beta-lactamase was obtained from S. lividans ...
Repository : ORBi (University of Liège) , View repository documents
Language(s) : English
Subject(s) : Cloning, Molecular* , DNA, Bacterial , Genes* , Nucleic Acid Hybridization , Streptomyces/enzymology , Streptomyces/genetics* , beta-Lactamases/genetics* , beta-Lactamases/metabolism , Life sciences :: Biochemistry, biophysics & molecular biology [F05] , Sciences du vivant :: Biochimie, biophysique & biologie moléculaire [F05] , Life sciences :: Microbiology [F11] , Sciences du vivant :: Microbiologie [F11]
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Source(s) : &ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&date=1987&jtitle=Journal+of+General+Microbiology&issn=0022-1287&volume=133&issue=10&pages=2915-2920&pub=Society+for+General+Microbiology&place=Reading&place=United+Kingdom
Publication Date(s) : 1987-10-01